DNA Vectors
Vector Biolabs pre-synthesized DNA vector
Protein tyrosine phosphatase non-receptor type 1 Adenovirus (Ad-PTP1B)
Vector Biolabs FLAT tagged CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus (Ad(RGD)-GFP-FLAG-hCas9)
The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This RGD-fiber modified adenovirus expresses a codon-optimized spCas9 nuclease with FLAG tag and a GFP reporter. It can be used along with guided RNA for genome-editing in cells that regular Ad5 doesn't infect well, including human or mouse macrophages, ES cells, T cells, synoviocytes, certain fibroblast and islet grafts etc.
Vector Biolabs v-raf-1 murine leukemia viral oncogene homolog 1 Adenovirus (Ad-RAF1)
The Raf kinases are important intermediates in signal transduction pathways. Raf-1, the prototype member of this gene family, is a 72-76 kDa cytoplasmic protein with intrinsic serine/threonine kinase activity, is broadly expressed in many cell types and is the cellular homolog of the viral oncogene, v-Raf. Other members of the Raf gene family include Raf-A and Raf-B. Raf-1 phosphorylates and thereby activates MEK (also designated MAP kinase kinase). Ras proteins bind Raf-1, but only when Ras is in its active GTP-binding state. This interaction results in Raf-mediated MEK activation. Two more recently described Raf-related proteins, Ksr-1 and Tak1 (TGFbeta-activated kinase), function as upstream regulators of Ras signaling pathways.
Vector Biolabs Protein kinase C, gamma Adenovirus (Ad-PKC-gamma)
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth, differentiation, gene expression, and hormone action. PKCs are protein serine/threonine kinases whose activities are dependent on calcium and phospholipids. PKCs can be subdivided into two major classes, including conventional (c) PKC isoforms and novel (n) isoforms. A more recently described member of this family has been designated either PKC µ for the human protein or PKD for isolates of mouse origin.
Vector Biolabs Complement component 1, q subcomponent, receptor 1 Adenovirus (Ad-C1qR(p))
Complement component 1, q subcomponent, receptor 1 Adenovirus (Ad-C1qR(p))
Vector Biolabs AAV-Cre (AAV serotype 5) AAV (AAV5-Cre)
This AAV serotype 5 virus (Capsid from AAV5 and ITR from AAV2) expresses Cre Recombinase under the control of a CMV promoter. This Cre contains a nuclear localization signal (NLS).Cre recombinase is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cultured cells or experimental animals.
Vector Biolabs human STK16 shRNA Adenovirus (Ad-h-STK16-shRNA)
This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human STK16 gene. The shRNA expression is driven by an U6 promoter.
The knockdown of this human gene was validated by qPCR in A549 cells.
Vector Biolabs Signal transducer/activator of transcription 5B Adenovirus (Ad-STAT5b)
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression.
Vector Biolabs AAV Serotypes Testing Kit AAV (AAV Testing Kit)
This kits contains AAV1-CMV-GFP, AAV2-CMV-GFP, AAV5-CMV-GFP & AAV6-CMV-GFP viral stock. It can be used for testing the infection efficiency on different cell types to see which serotype works best.We also customize an AAV serotype testing kit with other serotypes of your choice (AAV 3, 8, 9, 10, DJ, DJ8, DJ9, etc), or with different promoters (CAG, SYN, etc). Please ask for customized testing kit using the link under "Add to Cart".
Vector Biolabs SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 Adenovirus (Ad-SMARCc1)
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
BIOHIPPO AAV-mscRE16-minBGpromoter-Flp-WPRE-hGH-polyA
Recombinant adeno associated virus expressing Flp under the mscRE16 promoter with WPRE and hGH polyA elements. The Cre and Flp logic configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
