DNA Vectors
ATCC HMC3 NFKB-LUC2
For-profit Organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Applications. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item. Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
SignaGen Laboratories AAV-Syn-FLPo (AAV Serotype 5) | 1 x >30 µL
FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.
APPLIED VIROMICS LLC PRE-MADE AAV-( AAV2-WT)
Concentration of 1E12GC/mL, in 100uL aliquots. All vectors are assayed for identity and titer
Sigma Aldrich Fine Chemicals Biosciences CAS9 Blasticidin Lenti Plasmid, 50 uL vial
This product is a blasticidin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Functional Genomics/Screening /Target Validation/Genome Editing.
System Biosciences LLC pGreenFire 2.0 NFAT Reporter Virus | >2 x10^6 IFUs
With the pGreenFire 2.0 NFAT Reporter Lentivector & Virus (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro) the core reporter functionality is similar to the original pGreenFire lentivector—NFAT transcriptional response elements (TREs) are placed upstream of a minimal CMV promoter (mCMV) which together drive co-expression of rFLuc and GFP in response to NFAT activity. The result is the ability to quantitatively measure NFAT activity using both fluorescence and luciferase activity.