DNA Vectors
IBA LifeSciences StrGate Acptr Vctr pCSG-IBA104
pCSG-IBA104 is a large expression vector with universal features for transient expression of target proteins with an N-terminal Twin-Strep-tag as well as for generation of stable mammalian cell lines. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and ColE1 origin for a high plasmid copy number. Extrachromosomal replication in mammalian cells could occur either by origin of replication from Epstein-Barr Virus (oriP) or by SV40 ori. For the former the vector provides the EBNA-1 gene and for the latter the cell line has to be latently infected with SV40 or express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Stable cell lines can be selected by the neomycin resistance gene (NeoR). In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression. The expressed protein is transferred into the medium due to the BM40 secretory signal peptide.
ABclonal Technology Rubicon Rabbit pAb
The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
System Biosciences LLC PCDH-MSCV-MCS-EF1A-PURO CDNA D
pCDH-MSCV-MCS-EF1α-Puro cDNA Dual Promoter Cloning and Expression Lentivector
