DNA Vectors
System Biosciences LLC pCDH-CMV-MCS-EF1α-RFP Cloning and Expression LentivectorpCDH-CMV-MCS-EF1α-RFP cDNA Dual Promoter Cloning and Expression Lentivector = >1E6 IFUs
With the strong CMV promoter upstream of an MCS and EF1α driving the RFP reporter, pCDH-CMV-MCS-EF1α-RFP is a great, all-purpose lentivector. Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the strong CMV promoter, and expression of the RFP reporter from the EF1α promoter.
System Biosciences LLC PGREENFIRE1-LXRE IN LXRA HEPG2 CELL LINE
Study cholesterol homeostasis by co-expressing dscGFP and luciferase in response to Liver X Receptor (LXR) activity at the LXRα promoter in HepG2 Cells. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGreenFire1-LXRE-in-LXRα Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from the Liver X Receptor (LXR) response elements (LXREs) and neighboring regions in the LXRα promoter paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of the LXRE/LXRα transcriptional elements, drives expression of dscGFP and luciferase in response to LXR activity. The result is the ability to quantitatively measure LXR activity at the LXRα promoter by fluorescence and luciferase activity.