DNA Vectors
BIOHIPPO AAV-D2-DIO-hChR2(H134R)-EYFP-WPREs
Recombinant adeno associated virus expressing hChR2(H134R) fused to EYFP under the D2 promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise optogenetic control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
BIOHIPPO AAV-GfaABC1D-Lck-EGFP-WPRE-SV40-polyA
Recombinant adeno associated virus expressing EGFP Lck-EGFP under the GfaABC1D promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
ATCC Raji-GFP-Luc2 (CCL-86-GFP-LUC2) Frozen Reporter Cell Line, Dual GFP and Luciferase (luc2), BSL 2, CD19 positive
Raji-GFP-Luc2 (CCL-86-GFP-LUC2) is a dual-reporter Raji (Burkitt lymphoma) cell line engineered to constitutively express green fluorescent protein (GFP) and firefly luciferase (luc2). Supplied frozen for research use, the cell line provides stable reporter expression and native CD19 antigen for in vitro CAR-T cytotoxicity and multimodal reporter assays.
- Dual-reporter expression of GFP and luciferase for fluorescent and bioluminescent assays.
- Suitable as a CAR-T target cell line for CD19 and CD20 cytotoxicity assays.
- Frozen format for long-term storage; store in vapor phase of liquid nitrogen.
- Biosafety level 2; handle under appropriate containment.
- Doubling time approximately 20 hours.
- Recommended culture in RPMI-1640 with 10% fetal bovine serum and puromycin selection at 0.4 μg/mL.
- Thaw rapidly in a 37°C water bath and culture at 37°C with 5% CO2.
ATCC NCI-H929-GFP-Luc2 (CRL-3580-GFP-LUC2) Human plasmacytoma cell line, GFP and Luc2 reporters, suspension
NCI-H929-GFP-Luc2 is a human plasmacytoma (myeloma) suspension cell line engineered to constitutively express green fluorescent protein (GFP) and firefly luciferase (luc2). It is intended for use as a target cell in immuno-oncology research, CAR-T cytotoxicity assays, and cell-based assay development.
- Human plasmacytoma (bone marrow) cell line.
- Expresses green fluorescent protein and firefly luciferase (luc2).
- Suitable as a target for CAR-T and immuno-oncology assays.
- Suspension growth suitable for cell-based killing assays.
- Recommended medium: RPMI-1640 with 10% fetal bovine serum and 0.05 mM 2-mercaptoethanol.
- Maintained under puromycin selection (0.06 μg/mL) to retain reporter expression.
- Cryopreserved frozen vials; store in vapor phase of liquid nitrogen.
BIOHIPPO Empty Vector CMV-V5/GFP/Puro
Empty Vector CMV-V5/GFP/Puro lentiviral vector expressing GFP under CMV promoter Each vial provides a nominal titer of 2x10E6 transducing units TU Lentiviral particles packaged with Empty Vector Ctrl ORF cDNA Lentivirus CMV promoter drives expression of fluorescent reporter and resistance marker separated by P2A An epitope tag remains upstream of the fluorescent reporter separated by T2A and would otherwise be C-terminal to the ORF cDNA which is not present in this empty vector control This product is great for use as a negative control for any of our ORF cDNA lentiviral particles You can choose the empty vector construct containing the epitope tag reporter and selection marker to match the ORF cDNA lentivirus product you are using The transduced cells will express all these components without any ORF cDNA insert Vector Layout 5 LTR-CMV-[no cDNA]-tag-T2A-Reporter-P2A-Selection-3 LTR
Medchemexpress LLC Msr-ratio (Msr-green) | 2290635-22-0 | 99.1% | 310.37 | C18H14O3S | 10 MG
Msr-Ratio (Msr-green) is a ratiometric fluorescent probe for monitoring methionine sulfoxide reductase (Msr) activity in vitro and in live cells. It exhibits excitation at 375 nm and emission at 550 nm, and is supplied at high purity for research applications.
- Ratiometric fluorescent probe for methionine sulfoxide reductase activity.
- Excitation 375 nm and emission 550 nm for sensitive detection.
- High purity (99.13%) suitable for cell-based and in vitro assays.
- Molecular weight 310.37 for compact chemical profile.
- Available in small quantities such as 10 mg for research workflows.
Abcam SpCas9 D10A Nickase (Active), 1MG
SpCas9 D10A Nickase (Active) is a Streptococcus pyogenes serotype M1 Full Length protein, in the 2 to 1368 aa range, expressed in Escherichia coli, with >=95% purity, < 10 EU/mg endotoxin level and suitable for SDS-PAGE, HPLC, Biological Activity.
Protein/ Peptide/ Enzyme Synonyms: csn1, SPy_1046, cas9, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9
Conjugation: Unconjugated
The product is subject to the following: Abcam Restricted Use Statement
IBA LifeSciences StrGate Acptr Vctr pASG-IBA102
The pASG-IBA102 vector is designed for expression of recombinant proteins with a C-terminal Twin-Strep-tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
BIOHIPPO AAV-OT-ArchT-EGFP-WPREs
Recombinant adeno associated virus expressing ArchT fused to EGFP under the OT promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
New England Biolabs, Inc. PhiX174 Virion DNA – 250 µg
ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70. It has a molecular weight of 1.70 x 10^6 daltons and is 5386 bases in length. Greater than 85% of the molecules are circular.