DNA Vectors
SignaGen Laboratories AAV-Syn-FLPo (AAV Serotype 5) | 1 x >30 µL
FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.
System Biosciences LLC PLL-EF1A-LUCIFERASE LENTIVECTRpLL-EF1a-Luciferase-T2A-Puro (Lenti-Labeler™ plasmid) = 10ug
pLL-EF1a-Luciferase-T2A-Puro Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus. SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-EF1α-Luciferase-T2A-Puro Lenti-Labeler™ construct expresses luciferase from the EF1α promoter, which delivers moderate expression in most cell types (including primary and stem cells), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL250PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL250VA-1), SBI’s pLL-EF1α-Luciferase-T2A-Puro Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster.
System Biosciences LLC pCMV-CRE, Cre Recombinase Expression PlasmidCre Recombinase Expression Plasmid (pCMV-CRE) = 10ug
Transiently express Cre recombinase to remove selection cassettes from your genome engineering projects—use with HR Targeting Vectors that contain LoxP sites. With the Cre-LoxP site-specific recombination system used in most of our HR Targeting Vectors, you can get rid of extra sequences, such as selection cassettes, by transiently expressing the Cre recombinase from the pCMV-CRE Cre Recombinase Expression Plasmid.
HORIZON DISCOVERY BIOSCIENCES LIMITED EDIT-R LENTIVIRAL HEF1A BLAST
NC1763224 EDIT-R LENTIVIRAL HEF1A BLAST
OriGene CLONING VECTOR PCMV6-XL4, 10UG
Cloning vector PCMV6-XL4, 10 ug circular vector Untagged Cloning Vector
ANTIBODY DESIGN LABS CM13d3
CM13d3 is a helper phage engineered for multivalent phage display. CM13d3 is a derivative of M13KO7 (1) having a wild-type pIII phenotype which allows efficient bacterial transduction but lacking a functional pIII gene thus driving the display of pIII-fusion proteins expressed by the phagemid on the phage head (2). The use of pIII-defective helper phage has been shown to increase display of scFv by a factor up to 100-fold and more (3-5). This preparation contains enough helper phage to superinfect up to 0.5 L (500 ml) of TG1 culture. Other bacterial strains may require different amounts of CM13d3 helper phage.
Wilson Wolf Manufacturing Corp G-REX 5M OPEN SYSTEM, STERILE
G-Rex 5M Open System, Sterile Fluid Path
System Biosciences LLC PLL-EF1A-LUCIFERASE-T2A-PUROpLL-EF1a-Luciferase-T2A-Puro (Lenti-Labeler™ virus) = >2E6 IFUs
pLL-EF1a-Luciferase-T2A-Puro Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus. SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable. SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-EF1α-Luciferase-T2A-Puro Lenti-Labeler™ construct expresses luciferase from the EF1α promoter, which delivers moderate expression in most cell types (including primary and stem cells), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use.
Eurofins Discoverx PH PROLABEL DETECTN KIT 800DP
The PathHunter® ProLabel Detection Kit is for use with PathHunter Cell Lines expressing ProLabel® (PL) or ProLink™ (PK) expression or cloning vectors. The kit measures the total amount of ProLabel- or ProLink-tagged protein expressed in cells. Functional assays using PathHunter Cell Lines expressing both a ProLabel- or ProLinktagged protein and EA require the PathHunter Detection Kit (93-0001). The assay can be used in both 96-well and 384-well microplate formats. 800 dp (384-well)
AB Vector LLC GC (Green Control), 1 mL
Positive control baculovirus in experiments where GFP fluorescence is used to monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks.
Spherotech EASYCOMP BEADS PE-TR 10(7) 1ML
SPHEROTM EasyComp Fluorescent Particles - EasyComp PE-TR, 10(7)/mL; Unit: 1 mL