DNA Vectors
Vector Biolabs b-gal/LacZ Adenovirus (Ad-CMV-b-Gal)
beta-galactosidase / LacZ recombinant adenovirus
Vector Biolabs Myeloid differentiation primary response gene (88) Adenovirus (Ad-Myd88)
Mammalian Toll-like receptors (TLRs) recognize conserved products of microbial metabolism and activate NFkB and other signaling pathways through the adapter protein MyD88. MyD88 functions as an adapter protein in the association of IL-1 receptor associated kinase (IRAK) with the IL-1 receptor. MyD88 contains a characteristic N-terminal death domain, which is essential for NF-kB activation, and an adjacent Toll/Il-1R homology domain (TIR domain), which is responsible for signal transduction. Mal (MyD88 adapter-like, also designated TIR domain-containing adapter protein (TIRAP), wyatt, or TLR-4 adaptor protein) is a cytoplasmic TIR-domain-containing protein that forms homodimers and also heterodimers with MyD88.
Vector Biolabs pre-synthesized DNA vector
Aryl hydrocarbon receptor nuclear translocator-like Adenovirus (Ad-bMAL1)
Creative Biogene PCOMB3XSS VECTOR
VPT4013 is the modified version of pComb3X. The "SS" refers to the double stuffer, a 1200bp stuffer in the Fab light chain cloning region bounded by SacI and XbaI restriction sites and a 300bp stuffer in Fab heavy chain cloning region bound by XhoI and SpeI restriction sites. Also, the 1600bp double stuffer (both stuffer plus the leader sequence between the Fab light chain and heavy chain cloning regions) can be removed by SfiI digest so that non-Fab genes of interest can also by cloned.
Expression Systems BestBac 2.0 V-Cath/ChiA Deleted Baculovirus DNA
Expression Systems popular BestBac Linearized Baculovirus DNA backbone has been engineered to deliver highly efficient recombination with all polyhedron gene locus based transfer vectors.
Unmatched productivity and stability for BEVS
Improved production methodology can reduce need for plaque purification
Produces high titers of 5 E8 to 3 E9 IU/mL of recombinant baculovirus in P1 stock
Generates P1 stock as quickly as Bac-to-Bac and other systems
Each BestBac vial contains enough material for a minimum of five cotransfections
Suitable for other expression systems including BacMam™
BestBac 2.0 is a deletion mutant of BestBac 1.0. The v-cath/chiA deletion removes a potentially detrimental protease and may reduce competition for resources for protein synthesis during late gene expression. The v-cath/chiA deletion can improve the integrity of your expressed protein resulting in a significant increase of functional protein yield.
Vector Biolabs Period homolog 3 (Drosophila) Adenovirus (Ad-PER3)
The Per-Arnt-Sim (PAS) domain was identified as a 270 amino acid motif that mediates associations among various PAS family transcription factors. PAS domain family members include period proteins (Per1, Per2 and Per3), Arnt 1 (also designated HIF-1?), Arnt 2, Ah Receptor, BMAL1 (for Brain and Muscle Arnt-like protein 1; also designated Arnt 3, TIC, JAP3 or MOP3), Clock, single-minded proteins (SIM1 and SIM2) and hypoxia-inducible factors such as HIF-1a (also designated MOP1), EPAS-1 (also designated HIF-2a, MOP2 or HLF) and HIF-3a. The PAS family also contains a neuronal specific transcription factor known as NPAS2 that is involved the development and maintenance of learning and memory pathways. NPAS2, which is also designated PAS 4/MOP4, associates with MOP3 to activate transcription.
Vector Biolabs neurogenic differentiation 1 Adenovirus (Ad-NeuroD1)
Neuro D (also designated BETA 2) is a basic helix-loop-helix (bHLH) transcription factor functioning in neurogenic differentiation. Neuro D is expressed in pancreatic islet cells and transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors, and Neuro D can convert presumptive epidermal cells into neurons. BETA 3 is a novel helix-loop-helix protein that can function as a negative regulator of both Neuro D and Myo D-responsive genes. BETA 3 exhibits the highest degree of sequence relatedness with Neuro D and is most highly expressed in lung, kidney and brain cells.
Vector Biolabs Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha Adenovirus (Ad-iKBa)
Members of the IkB family can be divided into four groups including IkB-alpha (including proteins previously referred to as pp40 and MAD-3), IkB-beta, IkB-gamma (a 70 kDa protein identical in sequence to the C-terminal domain of the p105 precursor of NFkB p50) and IkB-epsilon. Members of the IkB family exhibit differential inhibition of the DNA binding activity of various NFkB homodimeric and heterodimeric complexes. A functionally related protein, Bcl-3, is overexpressed in chronic lymphocytic leukemia.
Vector Biolabs AAV-CreERT2 (AAV serotype 1) AAV (AAV1-CreERT2)
A mutated form of estrogen ligand-binding domain (ERT2) that binds to synthetic antagonists (such as tamoxifen or its derivative 4-hydroxy-tamoxifen) but not to circulating estrogens was fused to the Cre recombinase (Cre) to create the CreERT2, which requires the presence of tamoxifen for Cre recombinase activity. Therefore it makes possible to induce the recombination in cells with tamoxifen.This AAV-1 virus expresses CreERT2 under a CMV promoter.
Vector Biolabs Akt2 Adenovirus (Ad-CMV-Akt2 (wt))
The Akt family of kinases includes Akt1, Akt2 and Akt3. An HA tag was fused in frame to the N termini of the mouse Akt2 coding sequences.