DNA Vectors
Vector Biolabs Chemokine (C-C motif) ligand 2 Adenovirus (Ad-MCP-1)
Chemokines are members of a superfamily of inducible, secreted, pro-inflammatory cytokines. Members of the chemokine family exhibit 20 to 50% homology in their predicted amino acid sequences and are divided into four subfamilies. In the C-X-C or alpha subfamily, the first two of four cysteine residues are separated by a single amino acid. In the subfamily designated C-C or beta, the first two cysteines are adjacent. C subfamily members, also designated gamma chemokines, lack the first and third cysteine residues of the conserved motif. Chemokines in these three subfamilies are small, secreted proteins with molecular weights ranging from 7-15 kDa. Fractalkine is a 373 amino acid protein that contains a novel C-X3-C motif in which the first two cysteines are separated by three amino acid residues.
Vector Biolabs mouse PKC-alpha shRNA Adenovirus (Ad-m-PKC-alpha-shRNA)
This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown mouse PKC-alpha gene. The shRNA expression is driven by an U6 promoter.
The knockdown of this mouse gene was validated by western blot in C2C12 cells.
Vector Biolabs AAV-Cre-GFP (AAV serotype 1) AAV (AAV1-Cre-GFP)
This AAV serotype 1 virus (with Capsid from AAV1 and ITR from AAV2) expresses both Cre recombinase (tagged with nuclear localization sequence) and eGFP marker. Each gene expression is driven by its own CMV promoter.Cre recombinase is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cultured cells or experimental animals.
Vector Biolabs AAV-Cre (AAV serotype 6) AAV (AAV6-Cre)
This AAV serotype 6 virus (Capsid from AAV6 and ITR from AAV2) expresses Cre Recombinase under the control of a CMV promoter. This Cre contains a nuclear localization signal (NLS).Cre recombinase is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cultured cells or experimental animals.
Vector Biolabs FOXO3A Adenovirus (Ad-GFP-FOXO3A)
This adenovirus contains both a wild type FOXO3A gene and a GFP as a marker. The Foxo3a has a HA tag.
Vector Biolabs aryl hydrocarbon receptor nuclear translocator Adenovirus (Ad-HIF1b/ARNT)
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1)
Vector Biolabs AAV-Cre (AAV serotype 2) AAV (AAV2-Cre)
This AAV serotype 2 virus (both Capsid & ITR from AAV2) expresses Cre Recombinase. This Cre contains a nuclear localization signal (NLS).Cre recombinase is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cultured cells or experimental animals.
Vector Biolabs CCAAT/enhancer binding protein (C/EBP), epsilon Adenovirus (Ad-C/EBP-Epsilon)
The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder.
Vector Biolabs Mitogen-activated protein kinase 9 Adenovirus (Ad-JNK2)
A distant member of the MAP kinase family, designated c-Jun N-terminal kinase (JNK1), is activated by dual phosphorylation at a Thr-Pro-Tyr motif (as compared to the Thr-Glu-Tyr phosphorylation motif characteristic of MAP kinases) during response to ultraviolet (UV) light. JNK1 functions to phosphorylate c-Jun at N-terminal serine regulatory sites, Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. Two additional members of the JNK family, JNK2 (also designated p54(alpha), MAPK9) and JNK3 (also designated p54(beta)), were described in a study in which an independent isolate of JNK1 was designated p54(gamma). These proteins are designated stress-activated protein kinases, or SAPKs. This is JNK2 alpha-2 isoform.
Vector Biolabs U6 Empty Adenovirus (Ad-U6-Blank)
Adenovirus with U6 promoter only. Used as RNAi control adenovirus.
Vector Biolabs cAMP responsive element binding protein 1 Adenovirus (Ad-CREB1)
The ATF/CREB family consists of a series of transcription factors that function by binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, LZIP (also designated CREB3 and Luman), CREM-2, ATF-1, ATF-2, ATF-3, ATF-4, ATF-6 and ATF-7. These transcription factors share terminal leucine zipper dimerization and basic DNA binding domains and are highly variable in their N-termini. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, they can also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. Protein kinase A-mediated CREB phosphorylation induces association with a 265 kDa nuclear protein designated CBP (CREB-binding protein), which may represent a CREB coactivator.
Vector Biolabs Retinoblastoma-like 2 (p130) Adenovirus (Ad-p130)
The retinoblastoma susceptibility protein Rb is a 110 kDa phosphorylated protein. Like p53, Rb p110 is an anti-oncogene that is subject to inactivation by either mutation or by binding to certain DNA tumor virus-encoded proteins. Rb binds to and regulates transcription factors involved in cell cycle regulation, including the E2F family. Two Rb related proteins, p107 and p130, also function to regulate specific members of the E2F transcription factor family. Binding and inactivation of E2F proteins by Rb is regulated by cyclin dependent kinase-mediated phosphorylation.
Vector Biolabs CRISPR/Cas9 nuclease Adenovirus (Ad-Cas9)
The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. This adenovirus expresses a codon-optimized NLS-spCas9 nuclease. It can be used along with guided RNA for genome-editing.
Vector Biolabs SMAD, mothers against DPP homolog 2 Adenovirus (Ad-SMAD2)
Mammalian homologs of the Drosophila Mad gene include Smad1 (also designated Madr1 or JV4-1), Smad2 (also designated Madr2 or JV18-1), Smad3, Smad4 (also designated DPC4), Smad5, Smad6, Smad7 and Smad8 (also designated Smad9). Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGFb and activin-mediated growth modulation. Smad4 is required for all of the above activities and necessitates FAST-1 for DNA binding. Smad6 and Smad7 regulate the response to activin/ TGF-beta signaling by interfering with TGF-beta-mediated phosphorylation of other Smad family members.