RNA Preparation and Purification
Research Products International Corp ZR-96 Quick-RNA Kit, 4 x 96 Preps
The Quick-RNA 96 Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin plate technology to yield high quality total RNA (including small RNAs ~17-200 nt) in about 30 minutes. The procedure is simple: Add the provided RNA Lysis Buffer to a sample, then purify the RNA using the provided Silicon-A Plate. The result is highly-concentrated, DNA-free RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencing etc.
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: Genomic DNA removal column and DNase I included.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Beckman Coulter Bind BBC Lysis Reagent (C42089)
Beckman Coulter Bind BBC (manufacturer part number C42089) is a lysis reagent in the Lysis LBE product series intended for nucleic acid extraction workflows. The product is listed by Beckman Coulter Life Sciences and by authorized distributors; detailed technical specifications and packaging information were not provided on available listings.
- Intended for lysis in nucleic acid extraction workflows.
- Part of the Lysis LBE product series.
- Available through authorized distributors.
Norgen Biotek Corp Plasma/Serum RNA Purification Midi Kit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a two column method. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µL to 1.5 mL. Plasma samples prepared from blood collected on heparin should not be used.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification and concentration are provided in one convenient and fast kit for the easy processing of large input volumes of bodily fluids.The purified plasma/serum RNA is fully compatible with all downstream applications including NGS and more
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (10 ug) - 10 preps
The Monarch Spin RNA Cleanup Kit (10 ug) rapidly and reliably purifies up to 10 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. The kit can also be applied for total RNA extraction from some sample types. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design helps ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 ul. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch Spin RNA Cleanup kits are also available for 50 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience
Zyagen Labs Rat ovary total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy Sprague Dawley RAT OVARY tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Norgen Biotek Corp RNA Clean-Up and Concentration Kit, 50 Prep
This kit is designed to clean-up and concentrate RNA (including miRNA) from TRIzol and TRI Reagent, enzymatic reactions, in vitro transcription, labelling reactions, etc. This is a robust kit for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications.The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.The kit elutes RNA in 20 µL for all types of downstream applications. For smaller elution volumes of 8 µL, please refer to the microspin column kits such as the RNA Clean-Up and Concentration Micro-Elute Kit for the highest concentration of RNA sample.
New England Biolabs, Inc. NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) – 24 reactions
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA.
New England Biolabs, Inc. NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) with Sample Purification Beads – 24 reactions
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA. For convenience, this kit includes RNA Clean beads.
Roche Diagnostics KAPA mRNA HyperPrep (96rxn) Kit
KAPA mRNA HyperPrep Kits include all reagents required for mRNA Capture, RNA fragmentation, cDNA synthesis, library preparation and amplification. KAPA Pure Beads are supplied in each kit for reaction cleanups while KAPA Adapters are available separately. KAPA mRNA HyperPrep (96rxn) Kit includes three component kits: mRNA Capture 96 rxn Kit, RNA Hyper 96 rxn Kit, and 3 x 5mL Pure Beads. *This item is non returnable.
Takara Bio NUCLEOSPIN RNA FLTR PLTE PK/4
NucleoSpin RNA Filter Plates (4) 96-well plates for filtration of cell and tissue homogenates pack of 4
Molecular Research Center BAN 100 ML
BAN is molecular biology grade 4-bromoanisole for phase separation in the TRI Reagent RT and RNAzol RT procedures.
American Research Products Inc Plant Ribulose 1 5 bisphospha
Plant Ribulose-1 5-bisphosphate carboXylase/oXygenase (RuBisCO) ELISA Kit from CUSABIO Detection Range 3 12 g/mL-800 g/mL Sensitivity 3 12 g/mL Sample plant tissues Method Competitive