RNA Preparation and Purification
Biochain Institute Inc Total RNA - Rat Normal Tissue: Bladder, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue 5 Donor Pool: Stomach, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Universal RNA - Dog Normal Tissues, 2x100 ug/PK
BioChain's Universal RNA is the total RNA prepared with diversified tissues to ensure the broadest coverage of genes. It can be used as controls for microarray study. The Universal RNA is made on an industrial scale to minimize the lot-to-lot variation.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Colon descending, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Zymo Research Corporation ZymoBIOMICS™ RNA Mini Kit (50 preps)
The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.
Biochain Institute Inc Total RNA - hypertension: Interventricular Septum, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Rat Normal Tissue: Adipose, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Epoch Life Science Inc GenCatch™ Plant RNA Purification System (50 preps) Uniquely formulated buffers, shearing tubes, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify total RNA from plant (and fungal) samples. Comes with shearing tubes for simple and fast homogenization and filtration of tissue. Total RNA with a molecular size greater than 200 nucleotides (which excludes smaller RNA such as 5S and 5.8S RNA, and tRNA) can be isolated. Silica membrane technology. Up to 100 ug yield. 50 ul elution volume. 100 mg sample size. Additional buffer provided for handling plant tissue that contains sticky secondary metabolites. RNA isolated can be used for a variety of molecular manipulations such as Northern blotting, RT-PCR, PolyA+ RNA selection, cDNA synthesis, Primer extension, in vitro translation, etc. No phenol/chloroform extraction nor ethanol precipitation required. Used by labs around the world. 18 month shelf life.
New England Biolabs, Inc. NEBNext Low-bias Small RNA Library Prep Kit - 24 reactions
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 ng - 1,000 ng) or enriched small (0.05 ng - 5 ng) RNA. The kit's reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries. This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample. For optimal flexibility in multiplexing, the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5), which must be purchased separately; 480 indices are available.
- Generates libraries with the lowest bias among commercially available kits
- Fastest workflow (~3.5 hours) for single-day libraries
- Multiplexable with up to 480 UDIs
- Simplified, gel-free workflow
- 18-month shelf life
Zyagen Labs Mouse C57 liver total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy C57BL/6 Mouse LIVER tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.