RNA Preparation and Purification
Research Products International Corp RNA Clean & Concentrator-5 w/Zymo-Spin IC Columns, Capped, 200 Preps
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
Research Products International Corp Quick-RNA MiniPrep Kit w/Zymo-Spin IIICG Columns, Capped, & Spin-Away Filters, 50 Preps
The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg). The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: Genomic DNA removal column and DNase I included.
New England Biolabs, Inc. 3'-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog – 1 umol
Blocking of the 3' -hydroxyl of m7G with 3' -0-Me assures that the capped transcripts are homogeneous. The 3' - hydroxyl of the non-methylated G is the only 3' - hydroxyl available for initiation. The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas.
Agilent Technologies High Sensitivity RNA Kit, 1000 samples, 200 to 6000 nt sizing range, 2 μL sample volume
The DNF-472-1000 High Sensitivity RNA Kit is designed for advanced analysis of total RNA and IVT mRNA with Agilent Fragment Analyzer systems. It provides precise RNA quality assessment, crucial for applications such as vaccine development, qPCR, and NGS. This kit allows for reliable sizing and quantification of RNA samples, ensuring its usefulness in various research applications.
- High sensitivity analysis optimized for low-concentration RNA samples.
- Accurate sizing from 200 to 6,000 nt with precise quantification.
- Objective quality assessment using RNA Quality Number (RQN).
- Compatible with Agilent Fragment Analyzer systems for efficient workflow.
- Requires only 2 μL of sample for testing.
Biochain Institute Inc Total RNA - Plant Normal Tissue: Orange, 100 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Zyagen Labs Human mammary total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy HUMAN MAMMARY tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Esophagus, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Diabetic Disease: Colon, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Macherey-Nagel NUCLEOSPIN 96 RNA BLD 96WL KIT
NucleoSpin 96 RNA Blood, 96-well kit for RNA from blood
Macherey-Nagel NUCLEOSPIN RNA CLEAN UP MXI KT
NucleoSpin RNA Clean up Maxi kit for RNA clean up and concentration
Omega Biotek Inc 2 mL Collection Tubes, 5000/cs
2.0 mL Tube, Capless, graduated. 100/pack, 50 pack/case.