RNA Preparation and Purification
New England Biolabs, Inc. NEBNext Ultra™ II RNA Library Prep with Sample Purification Beads – 24 reactions
The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg. For convenience, this kit includes SPRISelect beads.
New England Biolabs, Inc. NEBNext Ultra™ II RNA First Strand Synthesis Module – 96 reactions
This module is part of the Ultra II Directional RNA and Ultra II RNA workflows, for Illumina- compatible RNA library construction. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1 g total RNA. The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming. The module is optimized for use with the NEBNext Ultra II Directional RNA Second Strand Synthesis Module or the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module, and is part of the Ultra II RNA directional or non-directional workflows. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1µg total RNA.
TRIBIOSCIENCE INC Ribosome RNA Purification Kit, 20 Tests
Tribioscience’s Ribosome RNA Isolation Kit provides a streamlined approach to isolating ribosome RNA from different tissues and cells. Kit size is 20 sample preparation
Zymo Research Corporation Quick-RNA™ MidiPrep Kit (25 Preps)
The Quick-RNA Midiprep Kit is designed for the easy, reliable, and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Clean-Spin™column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ Midiprep Kit allows for the efficient recovery of total RNA from 103 to 108 cells or tissue. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
System Biosciences LLC EVERY EV RNA ISOLATION KIT = 20 Preps
Obtain high yields of total EVery, including small RNAs, from already isolated Exosomes. The easy 30-min protocol delivers highly pure, highly concentrated RNA. EV samples vary drastically in their purity and concentration. Some EV samples from sera of diseased subjects and plasma bring up the challenge of heavy load of contaminating proteins that can potentially lead to column clogging thus lower yields. New column dimensions work just as smoothly for RNA isolation from all types of EV samples, irrespective of upstream EV isolation methods.
New England Biolabs, Inc. NEBNext Ultra™ II RNA First Strand Synthesis Module – 24 reactions
This module is part of the Ultra II Directional RNA and Ultra II RNA workflows, for Illumina- compatible RNA library construction. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1 g total RNA. The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming. The module is optimized for use with the NEBNext Ultra II Directional RNA Second Strand Synthesis Module or the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module, and is part of the Ultra II RNA directional or non-directional workflows. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1µg total RNA.
Macherey-Nagel NucleoSpin RNA Columns, 50 columns
NucleoSpin RNA Columns (50) binding columns for the isolation of RNA pack of 50
New England Biolabs, Inc. NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® – 96 reactions
The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg.
- Generate the highest yields of high quality libraries, with a broad range of input amounts
- Increase the complexity and transcript coverage of your libraries
- Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility
- Rely on robust performance, even with low quality RNA, including FFPE
Qiagen, Inc. RNEASY LIPID TISSUE MINI KIT
RNeasy Lipid Tissue Mini Kits, Contents: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 mL and 2 mL), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers, Sample Size: 10 to 100mg, Preparation Time: 45 min., Binding Capacity: Upto 100
Research Products International Corp Quick-RNA MiniPrep Plus Kit w/Zymo-Spin IIICG Columns , Capped, & Spin-Away Filters, 200 Preps
The Quick-RNA Miniprep Plus Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from cells, all tissue types, whole blood, and biological fluids. The provided DNA/RNA Shield stabilizes samples, allowing them to be stored without the need for immediate freezing or processing for up to one month. Furthermore, DNA/RNA Shield inactivates RNases as well as microbial pathogens (viruses, bacteria, etc.). The procedure combines a unique buffer system with Zymo-Spin Column technology to yield high quality total RNA (including small RNAs 17-200 nt). Simply add DNA/RNA Shield and Proteinase K to extract total RNA from any tissue, then purify the RNA using the Zymo-Spin Column. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing, etc. In addition, the kit can be used for the enrichment of small and large RNAs in two separate fractions.
Research Products International Corp Zymo Direct-zol RNA MicroPrep with TRI Reagent, 200 Preps
The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis.