RNA Preparation and Purification
CEPHAM LIFE SCIENCES INC Hybridization Buffer, DNA/RNA – 500 mL
Hybridization Buffer, DNA/RNA – 500 mL
Zyagen Labs Rat heart, whole total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy Sprague Dawley RAT HEART, WHOLE tissues, treated with DNase and dissolved in water.RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Norgen Biotek Corp RNA Clean-Up and Concentration Kit, 96-well plate format, RNA Input up to 50 μg, Elution Volume 75 μL, Spin column chromatography, For total RNA including miRNA
RNA Clean-Up and Concentration Kit, 96-well plate format, RNA Input up to 50 μg, Elution Volume 75 μL, Spin column chromatography, For total RNA including miRNA
Roche Diagnostics LIGHTCYCLER RNA MASTER SYBR GR
LightCycler RNA Master SYBR Green I - 1 kit (for 96 reactions of 20 uL final reaction volume)
New England Biolabs, Inc. Monarch® Total RNA Miniprep Kit - 50 preps
The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Total RNA, including viral RNA, can also be extracted from clinically relevant samples like saliva and swabs. Cleanup of enzymatic reactions or purification of RNA from TRIzol-extracted samples is also possible using this kit. Purified RNA is highly pure, has minimal residual gDNA and ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, RNA-seq, Northern blot analysis, etc.
Roche Diagnostics KAPA RNA HyperPrep Kit with RiboErase (HMR) Globin, 96 Libraries, RNA-Seq, 25 ng - 1 μg input RNA, for high- and low-quality RNA, including FFPE tissue
The KAPA RNA HyperPrep Kit with RiboErase (HMR) Globin is a streamlined, strand-specific solution designed for high-quality RNA-seq library preparation from various RNA inputs. This kit reduces preparation time and combines enzymatic steps while ensuring comprehensive transcriptome coverage by efficiently depleting ribosomal RNA and globin mRNA from samples. It is suitable for both manual and automated workflows on Illumina platforms and is compatible with degraded samples, making it an ideal choice for various research applications.
- Streamlined library preparation reduces time
- Strand-specific RNA-seq libraries
- Compatible with low-input and degraded samples
- Supports both manual and automated workflows
- Efficient rRNA and globin mRNA depletion
- High throughput and consistent results
- Designed for Illumina platforms
- Kit accommodates 96 libraries
- Research use only