When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Imtechella halotolerans is Gramnegative and Allobacillus halotolerans is Gram-positive while, Truepera radiovictrix is resistant to lysozyme lysis and has very high GC content. These species represent different challenges in NGS-based analysis. Moreover, with accurately quantified cell number and a log abundance distribution, this standard enables absolute cell number quantification in cases such as pathogen load detection.