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Nucleotides are composed of a nitrogenous base covalently bound to a 5-carbon sugar molecule and one or more phosphate functional groups. They are the molecules that make up the structural units of DNA and RNA.
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Digoxigenin-11-dUTP is a modified nucleotide analog designed for incorporation into DNA or cDNA by enzymatic processes substituting for natural dTTP The digoxigenin moiety attached via an 11-atom linker to the C5-position of the uridine base facilitates recognition by anti-digoxigenin antibodies labeled with horseradish peroxidase alkaline phosphatase or fluorophores Digoxigenin-11-dUTP is enzymatically incorporated using methods such as PCR amplification nick translation primer extension 3 -end labeling and reverse transcription Based on these properties Digoxigenin-11-dUTP is primarily utilized in probe-based assays sequencing and fluorescent or chromogenic in situ hybridization (ISH) applications
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