Protein Labeling Reagents
Vector Laboratories Concanavalin A (Con A) Fluorescein 25 mg
Concanavalin A (ConA) recognizes α-linked mannose residues present as part of a "core oligosaccharide" in many serum and membrane-bound glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer, but below pH 5.6, it dissociates into dimers of 52 kDa. Derivatizations can produce stable forms with dimeric structures. Con A requires calcium or manganese ions at each binding site, so avoid using buffers that bind calcium (such as phosphate) to dilute Con A since a gradual loss in activity may occur. This fluorescein-labeled Con A features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is a mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside.
Dojindo Molecular Technologies Inc DAPGreen–Autophagy Detection(5 nmol)
DAPGreen is used to detect autophagy in live cells. Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. The small fluorescent molecule DAPGreen is used to detect autophagosomes and autolysosomes. The mechanism has been suggested to be that the dye is incorporated into the autophagosome during double-membrane formation via structural features and then emits fluorescence under hydrophobic conditions (λex: 450 nm, λem: 535 nm). DALGreen is cell-permeable, has no requirement of transfection method, and enables live-cell imaging with fluorescence microscopy, plate reader, and flow cytometry. For monitoring only autolysosomes, we recommend DALGreen [D675], as it allows the detection of phagosome-lysosome fusion. Dojindo offers various reagents for autophagy research. (https://www.dojindo.com/autophagy-research/#jmp-b)
Sigma Aldrich Fine Chemicals Biosciences Phalloidin Atto 565 suitab
Atto 565 is a novel fluorescent label that belongs to the class of Rhodamine dyes. It shows a strong absorption high fluorescence quantum yield high thermal and photostability and a very little triplet formation. Atto 565 consists of a mixture of two isomers with practically identical optical absorption and emission Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind f actin in liver and muscle cells. As a result of binding phalloidin actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action at high dose levels death of mice or rats occurs within 1 or 2 hours. fluorescent conjugates of phalloidin are used to label actin filaments for histolo
Abcam 6-p-Toluidino-2-naphthalenesulfonic acid, potassium salt (TNS-K+), 100MG
MW 351.5 Da, Purity >98%. Fluorescent probe for measuring protein conformational changes and quantitative measurement of protein hydrophobicity processes that modify the exposure of the probe to water. Essentially nonfluorescent in water. Fluorescent when bound to membranes (q.y. ~0.27) or hydrophobic domains of proteins (q.y. ~0.72). Fluorescence enhanced by cyclodextrins. Can be used to provide a sensitive method for separating and analyzing cyclodextrins by capillary electrophoresis.
The product is subject to the following: Abcam Restricted Use Statement
American Research Products Inc CHROMOGENIC BIOTIN LABELING KI
5000265691 CHROMOGENIC BIOTIN LABELING KI
HIMEDIA Bolton Selective Supplement, FD231, Microbiology Culture Media, Selective Supplement, Antibiotic Supplement, Campylobacter Enrichment, Lyophilized, Vials, 5 vials
Bolton Selective Supplement is a lyophilized antibiotic supplement for the selective enrichment of Campylobacter species from foods. Each vial, sufficient for 500 ml of medium, supplies cefoperazone, vancomycin, trimethoprim and amphotericin B, a combination that suppresses competing bacteria and fungi so injured Campylobacter can resuscitate and grow when added to Bolton Broth Base. Rehydrate with 50% aqueous ethanol before use. A key component of standard Campylobacter detection. Store at 2 to 8 C. Packaged as 5 vials.
American Research Products Inc WATER SOLUBLE BIOTIN LABELING
5000265802 WATER SOLUBLE BIOTIN LABELING