Protein Labeling Reagents
QA Bio Inc QA-Bio, Inc. • LudgerTag Monosaccharide Analysis Kit
Monosaccharide Analysis Kit • The LudegrTag Monosaccharide Analysis Kit Contains Reagents for the Release and Fluorescent Labeling of Monosaccharides from Glycoproteins.
LICOR IRDye 750 NHS Ester (0.5mg)
IRDye 750 NHS Ester is an infrared dye with detection near 750 nm. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins. This dye is not suitable for use with LI-COR Odyssey Imaging Systems.
STEMCELL Technologies GloCell™ Fixable Viability Dye Violet 510, Size: 100 Tests
GloCell™ Fixable Viability Dye Violet 510 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Violet 510 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 510 nm that can be detected using the 525/50 band pass filter used to detect AmCyan.
Abcam 2-NBD-Glucose, Fluorescent glucose uptake probe, 1MG
MW 342.26 g/mol. Fluorescently-labeled deoxyglucose analog, used to directly monitor glucose uptake by living cells and tissues. Also used as a topical contrast reagent for the detection of neoplasia. Uptake can be monitored by real-time confocal, high-resolution or wide-field fluorescence microscopy and flow cytometry.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Metho x y- x 04, amyloid beta fluorescent marker, 5MG
MW 344.4 Da, Purity >98%. Blood-brain barrier permeable amyloid β fluorescent marker. Blue fluorophore. Derivative of Congo Red (ab145645) and Chrysamine G. Selectively binds fibrillar β-sheet deposits with high affinity (Ki = 26.8 nM). Detects plaques, tangles and cerebrovascular amyloid in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Biotium MemBrite™ Fix 594/615 Cell Surface Staining Kit, 100 Reactions
MemBrite™ Fix Cell Surface Staining Kits are designed for covalently staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments. MemBrite™ Fix are new covalent cell surface stains that react irreversibly with cell surface proteins for staining that can withstand formaldehyde or alcohol fixation, and detergent permeabilization. Unlike lectins such as WGA, which bind specific targets that may vary between cell types, MemBrite™ Fix dyes react widely with cell surface proteins. MemBrite™ Fix staining is rapid and non-toxic to cells, and because MemBrite™ Fix dyes are highly water soluble, they stain cells much more evenly than traditional lipophilic membrane dyes like DiO, DiI, PKH, Vybrant, or CellMask™. This trial size kit has reagents sufficient for 100 labeling reactions. The kit contains 1 vial of MemBrite™ Fix 594/615 that is sufficient for 20 uL of 1000X dye after DMSO reconstitution.
Dojindo Molecular Technologies Inc PlasMem Bright Green dye (100 ul) offers plasma membrane staining for 24 hrs+ with clear visualization, high retentivity, low toxicity, water-soluble, no washing, applicable to live and fixed cells.
The plasma membrane consists of a lipid bilayer separating the intracellular environment from the extracellular space. Thus, the PM plays a central role in many cell behaviors such as cell migration, cell stretching, and signaling cascades. Additionally, PM dysfunction is an important biomarker because it is related to cell status and is linked to many diseases. PlasMem Bright dyes have higher retentivity on the plasma membrane. Furthermore, PlasMem Bright dyes are more water-soluble compared with other commercially available dyes and can be diluted with the culture medium. The PlasMem Bright dyes offer two different color options (green and red) and provide ready-to-use DMSO solutions. A working solution can be prepared easily via a single dilution step using growth medium or HBSS. Dojindo offers various reagents for cell membrane research. (https://www.dojindo.com/track-the-dynamics-of-plasma-membrane/)
Dojindo Molecular Technologies Inc DAPGreen–Autophagy Detection(5 nmol)
DAPGreen is used to detect autophagy in live cells. Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. The small fluorescent molecule DAPGreen is used to detect autophagosomes and autolysosomes. The mechanism has been suggested to be that the dye is incorporated into the autophagosome during double-membrane formation via structural features and then emits fluorescence under hydrophobic conditions (λex: 450 nm, λem: 535 nm). DALGreen is cell-permeable, has no requirement of transfection method, and enables live-cell imaging with fluorescence microscopy, plate reader, and flow cytometry. For monitoring only autolysosomes, we recommend DALGreen [D675], as it allows the detection of phagosome-lysosome fusion. Dojindo offers various reagents for autophagy research. (https://www.dojindo.com/autophagy-research/#jmp-b)
Sigma Aldrich Fine Chemicals Biosciences 6-(2,2-Dicyanovinyl)-N-(2-hydroxyethyl)-1,2,3,4-tetrahydroquinoline | 142978-25-4 | MFCD00274418 | 5MG
6-(2,2-Dicyanovinyl)-N-(2-hydroxyethyl)-1,2,3,4-tetrahydroquinoline | Mol Wt: 253.3 | 142978-25-4 | MFCD00274418 | 5MG
