Desalting Columns
Desalting Columns
Disposable or reusable gel filtration chromatography columns for use in protein biology or DNA extraction and purification, for the fractional purification of proteins, nucleic acids, and other macromolecules from low molecular-weight buffer salts and reagents.
Desalting columns are special kinds of gel filtration (exclusion) chromatography columns suitable for the fractional purification of proteins, nucleic acids, and other macromolecules. Their use offers a more convenient and quicker alternative to dialysis.
Desalting columns may be disposable or reusable plastic or glass columns prepacked with beads of a suitable media, typically polysaccharide (Sephadex), or porous polyacrylamide that are more resistant to enzymatic degradation. The beads have defined molecular weight cut-off (MWCO) exclusion points (typically around 6000 Da) that trap, retain, and slow down the elution of small molecules below the MWCO, but exclude and therefore do not impede the more rapid elution of larger macromolecules such as proteins.
Desalting columns can achieve high sample recovery yields of >95%, with column capacities ranging from 5 μL to 10 mL that are suitable for applied sample volumes anywhere between 2 μL and 4 mL.
Types of Desalting Columns
• Columns designed for simple gravity flow
• Spin columns designed especially for centrifugation
• Pressure-compatible columns designed for use with suitable pumps or with integrated preparative LC systems
How Are Desalting Columns Used?
• Removal of salts from protein samples
• Buffer exchange prior to downstream electrophoresis or affinity chromatography
• Removal of unreacted radioactive labels
• Removing phenol or free nucleotides from nucleic acid preparations
• Removing of primers or dyes from PCR or sequencing reactions
• Removal of other low molecular weight compounds such as cross-linking, labelling or derivatization reagents, cofactors, inhibitors, or other contaminants
Thermo Scientific™ Pierce™ Dextran Desalting Columns, 5K MWCO, 10 mL
Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with crosslinked dextran-based gel-filtration columns.
Thermo Scientific™ Polyacrylamide Desalting Columns, 6K MWCO, 10 mL
Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with porous polyacrylamide-based gel-filtration columns.
Thermo Scientific™ Zeba™ Spin Desalting Columns, 40K MWCO, 10 mL

Effectively remove salt and other molecules (40K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.
Thermo Scientific™ Zeba™ 96-well Spin Desalting Plates, 40K MWCO
Perform high-throughput sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening and assay development.
Thermo Scientific™ Zeba™ Spin Desalting Columns, 7K MWCO, 0.5 mL

Effectively remove salt and other molecules (7K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.
Thermo Scientific™ Pierce™ Polyacrylamide Desalting Columns
Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with porous polyacrylamide-based gel-filtration columns.
Thermo Scientific™ Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75µL
Effectively remove salt and other molecules (7K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.
Thermo Scientific™ Zeba™ Desalting Chromatography Cartridges, 7K MWCO, 1 mL
Rapidly desalt samples with high protein recovery with these ready-to-use cartridges for manual or automated liquid chromatography (LC).
Thermo Scientific™ Pierce™ Peptide Desalting Spin Columns
Desalt, clean up and prep 5μg to 5 mg peptide samples for mass spectrometry with these ready-to-use microcentrifuge columns.
Thermo Scientific™ Pierce™ Dextran Desalting Columns, 5K MWCO, 5 mL
Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with crosslinked dextran-based gel-filtration columns.
Thermo Scientific™ Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL

Effectively remove salt and other molecules (7K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.
Thermo Scientific™ Zeba™ Spin Desalting Columns, 7K MWCO, 10 mL

Effectively remove salt and other molecules (7K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.
Thermo Scientific™ Zeba™ Micro Spin Desalting Columns, 40K MWCO, 75 µL
Effectively remove salt and other molecules (40K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.
Thermo Scientific™ Pierce™ Polyacrylamide Desalting Columns, 1.8K MWCO, 5 mL
Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with porous polyacrylamide-based gel-filtration columns.
Thermo Scientific™ Zeba™ Spin Desalting Columns, 40K MWCO, 5mL

Effectively remove salt and other molecules (40K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.
Thermo Scientific™ Zeba™ Spin Desalting Columns, 40K MWCO, 0.5 mL
Effectively remove salt and other molecules (40K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.
Thermo Scientific™ Zeba™ Spin Desalting Columns, 40K MWCO, 2 mL
Effectively remove salt and other molecules (40K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.
Thermo Scientific™ Zeba™ Spin Desalting Plates, 7K MWCO
Perform high-throughput sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening and assay development.
Thermo Scientific™ Zeba™ Spin Desalting Columns, 7K MWCO, 2 mL

Effectively remove salt and other molecules (7K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.
Cytiva Disposable PD-10 Columns

Use for gravity flow purification. Disposable PD-10 Columns are economical, plastic columns that can be packed with a wide range of bulk media.
Promotions are available
Cytiva HiTrap™ Desalting Prepacked Columns

For rapid convenient desalting and buffer exchange. HiTrap™ Desalting Prepacked Columns are prepacked, ready to use column for group separation between high and low molecular weight substances.
Promotions are available
Cytiva Superdex 75 Increase Prepacked Column, 10/300 GL

Use these columns in preparative purification as well as for characterization and analysis of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva HisTrap™ excel Prepacked Columns feature tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
Promotions are available
Cytiva Superdex 75 Increase Prepacked Column, 3.2/300

Use this column for rapid purity check and screening of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva Superdex 75 Increase Prepacked Columns are ideal when time is limited and low buffer and sample consumption is important.
Promotions are available
Cytiva Superdex 75 Increase Prepacked Column, 5/150 GL

Use this column for rapid purity check and screening of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva Superdex 75 Increase Prepacked Columns are ideal when time is limited and low buffer and sample consumption is important.
Promotions are available
Cytiva Disposable PD-10 Desalting Columns

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.
Promotions are available
Cytiva HiPrep™ 26/10 Desalting Columns

Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15mL per column. Cytiva HiPrep 26/10 Desalting Column is a convenient, easy-to-use column prepacked 53mL column with Sephadex G-25 Fine for desalting and buffer exchange.
Promotions are available
Cytiva PD SpinTrap™ G-25 Desalting Columns
Use for convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr). Cytiva Life Sciences™ PD SpinTrap™ G-25 Desalting Columns provide the option of running multiple samples in parallel.
Promotions are available
Enzo Life Sciences Desalting Column and Resin (1 Set)
5 ml polypropylene disposable column and 1 g P6 desalting resin. Shipping: Ambient

Optimize Technologies OPTI-TRAP™ Micro Trap Cartridge, Protein, 5µL, 1mm x 8mm, 6pk

The OPTI-TRAP™ is a bi-directional trap cartridge system which is used individually or in a series for sample concentration and purification. The OPTI-TRAP™ system features a durable biocompatible trap holder assembly made of either PEEK (capillary configuration) or stainless steel with a PEEK flow path (micro and macro configurations). OPTI-TRAPs feature low back pressure and are loaded and eluted manually with a syringe or on-line when plumbed into the sample injection valve. Additionally OPTI-TRAPs include titanium frits for full biocompatibility and custom packing is also available upon request. The cartridges are available in three different sizes: 50uL, 5.0uL & 0.5uL and can be purchased as singles or in 6 packs.
