NP40 Lysis Buffer (High Salt) is a powerful reagent used in protein extraction, especially for breaking down cell membranes and releasing intracellular proteins. This 250mL volume of NP40 lysis buffer is designed for use in biochemical and molecular biology applications where high salt conditions are necessary for maintaining protein stability. The buffer is especially effective for solubilizing membrane proteins and extracting nucleic acids while preventing proteolysis. NP40 is commonly used in studies involving protein analysis, western blotting, and immunoprecipitation. It is also used in tissue culture and cell lysate preparation for downstream analyses, ensuring high-quality protein extracts for further research.