Reagents, kits, and instruments for use during the extraction of proteins from bacteria, cultured mammalian cells and tissues, insect cells, yeast cultures, flowering plants, etc. Products include lysis buffers, master mixes, and solubilizing solutions.
NativeLyser is a bacterial cell lysis buffer formulated to promote the efficient breakdown of bacterial cells while solubilizing and stabilizing the released recombinant proteins NativeLyser contains a proprietary mix of stabilizers that prevent the loss of recombinant proteins during the preparation of cell-free extracts By preventing denaturation proteolytic degradation and non-specific protein aggregation NativeLyser creates a protein-friendly environment when recombinant proteins are released from the bacterial cytoplasm We recommend the use of NativeLyser to purify proteins expressed using the NativeFolder Bacterial Culture Medium (Catalog number K1010004) NativeLyser is provided as a 5X concentrated solution (200 mL) that can be diluted with di-water to produce 1L of ready-to-use buffer For in vitro research use only
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>b>Enzymatic lysis of gram-negative bacteria. Gram-negative bacteria are less susceptible than gram-positive bacteria to lysoyme and detergents. Enzymatic lysis of gram-negative bacteria requires the outer membrane to be permeabilized to expose the peptidoglycan layer to attack. The Bacillus lysis buffer kit provides the buffer and additive needed for the enzymatic lysis of gram-negative bacteria.
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Supplied as a component of the Monarch HMW DNA Extraction Kit for Cells &Blood, the Monarch RBC Lysis buffer is specifically designed to lyse red blood cells (erythrocytes) while leaving leukocytes intact. The separate RBC lysis step enables removal of hemoglobin to increase the purity of the blood prep. The RBC Lysis Buffer also removes nucleases to enable the subsequent purification of HMW DNA into the megabase (Mb) range in size. This product should be stored at 4C.
A component of the Monarch HMW DNA Extraction Kit for Cells & Blood
Lyses red blood cells (erythrocytes) while leaving leukocytes intact
Enables removal of hemoglobin and nucleases in blood preps
Store Monarch RBC Lysis Buffer at 4°C
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More