Protein Resins
AdipoGen Progranulin human rec
Protein. Signal peptide and human progranulin aa 1-593 are fused at the C-terminus to a FLAG -tag. Source HEK 293 cells. Endotoxin content <0.01EU/µg purified protein LAL test Lonza. Lyophilized. Contains PBS. Purity >98% SDS-PAGE. Progranulin PGRN is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration FTLD. In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer‘s Disease AD and potentially for MCI Mild Cognitive Impairment. Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.
AdipoGen ST2 humanFc human rec
Protein. Signal peptide and the sequence of isoform 2 of human ST2 aa 1-328 are fused at the C-terminus to the Fc portion of human IgG1. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS, pH 7.2. Binds to human IL-33. Purity >90% SDS-PAGE. The ST2 Interleukin-1 receptor-like 1 Interleukin-33 receptor gene was originally identified as a gene induced by serum or oncogene expression in fibroblasts. The gene produces a shorter soluble secreted form ST2 and a longer, transmembrane form ST2L by alternative splicing. Soluble ST2 has been shown to downregulate the expression of TLR1 and TLR4. ST2L negatively regulates TLR4 signaling and induces endotoxin tolerance, and enhances Th2 responses. IL-33 is the specific ligand for ST2L.
AdipoGen GITRL Soluble mouse rec
Protein. The extracellular domain of mouse GITRL aa 45-173 is fused at the N-terminus to a FLAG -tag. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in 10mM glycine, pH 10.0. Binds to mouse GITR. Purity >90% SDS-PAGE. GITRL Glucocorticoid-induced TNF receptor ligand is expressed on dendritic cells DC, monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues e.g. testis. GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T cell proliferation and TCR-mediated apoptosis.
AdipoGen Jagged-2 hFc h rec
Protein. Human Jagged-2 aa 1-1054 is fused at the C-terminus to the Fc portion of human IgG. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Lyophilized. Contains PBS 0.02 % CHAPS. Purity >90% SDS-PAGE. Jagged-2 is a putative Notch ligand involved in the mediation of Notch signaling, that can induce stromal cells to secrete IL-6, VEGF and IGF-1. Notch activation can interact with NF-kappaB and C-myc to promote the proliferation and to inhibit the apoptosis of MM cells, showing in the relationship between the incidence of myeloma and drug resistance. Jagged-2 is also involved in limb development.
AdipoGen ANGPTL4 mouse rec
Protein. Mouse ANGPTL4 aa 1-410 is fused at the C-terminus to a FLAG -tag. Source COS-7 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS. Purity >90% SDS-PAGE. ANGPTL4 Angiopoietin-like protein 4 mainly expressed in endothelial cells hypoxia-induced. Regulates angiogenesis and modulates tumorigenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain FLD. Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase LPL converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions.
AdipoGen FGF-23 mouseFc h rec
Recombinant protein. Mouse FGF-23 aa25-251 is fused at the C-terminus to the Fc portion of human IgG1. Activates ERK and FRS2alpha phosphorylation in Klotho expressing cells. Source/Host HEK 293 cells. Purity >90% SDS-PAGE. Lyophilized. Contains PBS. FGF-23 Fibroblast growth factor 23 is a regulator of phosphate homeostasis. It upregulates EGR1 expression in the presence of KLBy. Acts directly on the parathyroid to decrease PTH secretion. Regulates the vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF-23 are the cause of autosomal dominant hypophosphataemic rickets ADHR and of hyperphosphatemic familial tumoral calcinosis HFTC.
AdipoGen Progranulin human rec
Protein. Signal peptide and human progranulin aa 1-593 are fused at the C-terminus to a FLAG -tag. Source HEK 293 cells. Endotoxin content <0.01EU/µg purified protein LAL test Lonza. Lyophilized. Contains PBS. Purity >98% SDS-PAGE. Progranulin PGRN is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration FTLD. In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer‘s Disease AD and potentially for MCI Mild Cognitive Impairment. Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.
AdipoGen ANGPTL7 human rec
Protein. Human ANGPTL7 aa 1-346 is fused at the C-terminus to a FLAG -tag. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS. Purity >90% SDS-PAGE. ANGPTL7 Angiopoietin-like protein 7 is expressed in various tissues and regulates angiogenesis. Human ANGPTL7 was characterized as a potent target gene of the WNT/beta-catenin signaling pathway, and is a pharmacogenomics target in the fields of oncology and regenerative medicine. Overexpression of ANGPTL7 increases collagen expression and might exert a pathogenic role in glaucoma.
AdipoGen RBP4 human rec
Protein. Human RBP4 aa 19-201 is fused at the N-terminus to a FLAG -tag. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS, pH 7.2. Purity >90% SDS-PAGE. Retinol binding protein 4 RBP4 RBP is a 21kDa secreted protein, a member of the lipocalin family and is known as the primary transporter of retinol vitamin A to tissues. A recent report revealed RBP4 as an adipokine linking glucose transporter 4 GLUT4 suppression in adipose tissue to insulin. Elevated human and mouse serum RBP4 levels are associated with insulin resistance and its severity, obesity, and certain components of metabolic syndrome. Furthermore, human serum RBP4 levels are closely related to renal function.
AdipoGen RBP4 human rec
Protein. Human RBP4 aa 19-201 is fused at the N-terminus to a FLAG -tag. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS, pH 7.2. Purity >90% SDS-PAGE. Retinol binding protein 4 RBP4 RBP is a 21kDa secreted protein, a member of the lipocalin family and is known as the primary transporter of retinol vitamin A to tissues. A recent report revealed RBP4 as an adipokine linking glucose transporter 4 GLUT4 suppression in adipose tissue to insulin. Elevated human and mouse serum RBP4 levels are associated with insulin resistance and its severity, obesity, and certain components of metabolic syndrome. Furthermore, human serum RBP4 levels are closely related to renal function.
Genscript Corporation Protein L Resin
GenScript Protein L Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The highly purified protein L ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein L Resin is greater than 15 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
PUROLITE COMPANY PRAESTO JETTED A50- 1L
Tricorn™ 5/50 packing equipment.•Tricorn™ 5/50 column. Glass filter funnelPlastic beaker Plastic spatula.Measuring cylinder Purified Water or 100 mM NaCl
Gold Biotechnology Inc Protein A Agarose Resin (Pharmaceutical grade for research) 100 mL
Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.