GenScript s Protein A Resin FF is an affinity chromatography media designed for easy one-step purification of classes subclasses and fragments of immunoglobulins from biological fluids and from cell culture media The recombinant protein A ligand is coupled to 4% highly cross-linked agarose The coupling is optimized to have a high binding capacity for immunoglobulins The static binding capacity of Protein A Resin FF is greater than 40 mg rabbit IgG/ ml settled resin The dynamic binding capacity will vary depending on several factors such as target antibody flow rate etc Table 1 lists the characteristics of Protein A Resin FF br br Protein A a bacterial cell wall protein isolated from i Staphylococcus aureus /i binds to mammalian IgGs mainly at their Fc region Native protein A has five IgG binding domains and many other domains with unknown functions Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce n