Class-Specific Protein Assay Kits
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New England Biolabs, Inc. Cold-Active TEV Protease - 1000 units
Cold-Active TEV Protease is an engineered version of the Tobacco Etch Virus (TEV) Protease. Cold-Active TEV Protease is optimized for higher activity at lower temperatures, improved thermal stability, and reduced autolysis. Cold-Active TEV Protease recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser/Ala/Met/Cys/His/Phe) and cleaves between the Gln and Gly/Ser/Ala/Met/Cys/His/Phe residues. This enzyme is often used for the removal of affinity purification tags such as maltose-binding protein (MBP-tag) or poly-histidine (His-tag) from recombinant proteins. Cold-Active TEV Protease has a 7xHis-tag for easy removal from a reaction by immobilized metal affinity chromatography (e.g. Ni Resin or Ni-NTA magnetic beads).
