Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Release Human Biotin Positive Selection Kit, Size: 1 x 10^9 cells
Immunomagnetic positive selection of particle-free human cells labeled with biotinylated antibodies
STEMCELL Technologies EasySep™ Human FITC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human FITC Positive Selection Kit II is designed to isolate cells that are labeled with FITC-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing FITC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human FITC Positive Selection Kit (Catalog #18552).
STEMCELL Technologies RoboSep™ Human CD33 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD33 Positive Selection Kit II is designed to isolate CD33+ cells from ammonium chloride lysed blood or bone marrow, or from nucleated cells prepared by density separation with RosetteSep™ DM-M density medium (Catalog #15725) by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 antigen is expressed on cells of the myeloid lineage.This product replaces the EasySep™ Human CD33 Positive Selection Kit (Catalog #18257) for even faster cell isolations.
STEMCELL Technologies RosetteSep™ Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail, For processing: 200 mL bone marrow processed
The RosetteSep™ Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail is designed to pre-enrich progenitor cells by removing lineage-specific cells from whole bone marrow by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3, CD11b, CD14, CD16, CD19, CD56, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The pre-enriched progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RoboSep™ Human Eosinophil Isolation Kit, For isolating: 1 x 10^9 cells
The EasySep™ Human Eosinophil Isolation Kit is designed to isolate eosinophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-eosinophils and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The kit is compatible with cells prepared using either Lymphoprep™ (Catalog #07801) centrifugation (preferred for higher cell purities) or HetaSep™ (Catalog #07906) sedimentation (preferred for faster, lysis-free sample processing).This product replaces the EasySep™ Human Eosinophil Enrichment Kit (Catalog #19256) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Rat IgM Depletion Kit, For processing: 1 x 10^9 cells
The EasySep™ Rat IgM Depletion Kit is designed to deplete IgM+ cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by positive selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies RoboSep™ Human Cord Blood CD34 Positive Selection Kit III, For processing: 1000 mL cord blood processed
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
STEMCELL Technologies EasySep™ Mouse PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This kit replaces the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554).For highly purified particle-free cells, we recommend the EasySep™ Release Mouse PE Positive Selection Kit (Catalog #17656). (https://www.stemcell.com/products/easysep-release-mouse-pe-positive-selection-kit.html)
Genscript Corporation CD8 alpha/CD8A His Human
CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I favoring lipid raft partitioning of TCR and inducing intracellular signaling CD8 alpha is also found on dendritic cells and rat macrophages but whether CD8 alpha enhances responses of a partner receptor like TCR to activate these cells is not known
STEMCELL Technologies RosetteSep™ Human NK Cell Enrichment Cocktail, For processing: 200 mL whole blood processed
The RosetteSep™ Human NK Cell Enrichment Cocktail is designed to isolate NK cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-NK cells and red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified NK cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
Lonza Walkersville Hu Naive CD8/CD45RA T-Cells
Na ve CD8 T cells are isolated using a two step isolation process CD8 T Cells are first negatively selected from MNC obtained from leukapharesed blood using an indirect immunomagnetic CD8 labeling system CD45RO microbeads are then used to deplete the CD45RO population leaving a highly enriched CD4 CD45RA Na ve T Cell population Cells are cryopreserved in Lonza Cryo Media and collected in a cryovial Cell purity is determined post thaw using a FACS assay Count and viability is determined using hemacytometer (trypan blue method) Cells are collected from healthy donors following IRB protocols
STEMCELL Technologies RosetteSep™ Human Cord Blood Debulking Cocktail, For processing: 40 mL cord blood processed
The RosetteSep™ Human Cord Blood Debulking Cocktail is designed to deplete lineage positive cells from fresh cord blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD14, CD19, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic stem and progenitor cells are present as a pre-enriched population at the interface between the plasma and the buoyant density medium.