Protein Purification Reagents and Kits
STEMCELL Technologies Mitochondrial Isolation Kit, Size: 50 Tests
Conveniently enrich the mitochondrial or cytosolic subcellular fractions from mammalian cells or tissues using the Mitochondrial Isolation Kit. This kit offers two quick options to isolate the mitochondrial subcellular fraction and has been verified for use with western blot analysis. The first option is a reagent-based method for processing up to six samples concurrently. The second option utilizes Dounce homogenization, providing a two-fold increase in isolated mitochondria from a single sample. Both options use a microcentrifuge-based method to separate the mitochondrial fraction from the cytosolic fraction while preserving their biological activity. This separation is a valuable tool for studying apoptosis and signaling events between the two fractions. For more information on the Mitochondrial Isolation Kit, visit www.stemcell.com.
Biolegend MojoSort on Columns - Large Columns Apps Cell Sep - Pos Cell Sep - Neg Size 25 Pack
MojoSort on Columns - Large Columns Apps Cell Sep - Pos Cell Sep - Neg Size 25 Pack
Invent Biotechnologies Inc Minute™ Protein Solubilization Reagent for MS (2 ml)
This item has a minimum qty of 4 per supplier requirements.
This reagent is formulated for dissolving protein pellets using protein kits from Invent Biotechnologies (Cat# Nos. SM-005, SM-005-P, CP-011, NE-013, YM-017, MM-018 and AF-023) and other companies. Solubilized proteins are denatured due to the presence of SDS and can be used for trypsin digestion and for subsequent mass spectrometry analysis.
STEMCELL Technologies EasySep™ Human Pan-DC Pre-Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Pan-DC Pre-Enrichment Kit is designed to pre-enrich all dendritic cells (DCs) (including myeloid and plasmacytoid DCs) from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-pan DCs and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Active Motif Histone Extraction Kit
The Histone Extraction Kit provides a simple method to acid precipitate histones from cell culture, tissue and primary cells (e.g. T-cells) while preserving histone post-translational modifications. The highly basic nature of histone proteins often precludes them from being isolated using traditional methods for protein extraction. Instead, histones must be isolated under acidic conditions to retain solubility. The inclusion of inhibitors helps to preserve histone modifications such as acetylation, methylation and phosphorylation. Each Histone Extraction Kit contains enough reagents for extractions from 2 x 96-well plates (192 rxns), or 100 rxns from cultured cell lines and tissues. Kits include buffers, protease inhibitors, phosphatase inhibitors, deacetylase inhibitors and optimized protocols for efficient recovery of core histones (H2A, H2B, H3 & H4). For more complete information, please visit the Active Motif website.
Hampton Research HR2-095/StockOptions Imidazole, pH 6.2 to 7.8 in 0.1 pH unit increments - 10 ml, tube format
HR2-095/StockOptions Imidazole, pH 6.2 to 7.8 in 0.1 pH unit increments - 10 ml, tube format. Please note items from this supplier are non-returnable.
Hampton Research SOL & STABILITY #70 (F10)
HR2-072-70/Solubility & Stability #70 (F10), 1.0 ml - 250 mM Sodium benzenesulfonate. Please note items from this supplier are non-returnable.
Hampton Research SOL & STABILITY #69 (F9)
HR2-072-69/Solubility & Stability #69 (F09), 1.0 ml - 250 mM Ammonium acetate. Please note items from this supplier are non-returnable.
Hampton Research SOL & STABILITY #68 (F8)
HR2-072-68/Solubility & Stability #68 (F08), 1.0 ml - 500 mM Lithium citrate tribasic tetrahydrate. Please note items from this supplier are non-returnable.
Hampton Research SOL & STABILITY #67 (F7)
HR2-072-67/Solubility & Stability #67 (F07), 1.0 ml - 500 mM DL-Malic acid pH 7.0. Please note items from this supplier are non-returnable.
Hampton Research SOL & STABILITY #84 (G12)
HR2-072-84/Solubility & Stability #84 (G12), 1.0 ml - 50% v/v Formamide. Please note items from this supplier are non-returnable.
Genscript Corporation Enterokinase, His, Bovine
Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.
Hampton Research SOL & STABILITY #83 (G11)
HR2-072-83/Solubility & Stability #83 (G11), 1.0 ml - 5% w/v Polyethylene glycol monomethyl ether 750. Please note items from this supplier are non-returnable.