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Spin Columns and Gravity-flow Columns

Purification columns and filter spin columns that are prepacked or designed to be packed with desalting resins, agarose beads, and other affinity supports for use in centrifuge/microcentrifuge or gravity-flow chromatography procedures.

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IBA LifeSciences Strep-Tactin TACS Agarose Column; 1 ml

Strep-Tactin TACS Agarose columns contain agarose macrobeads coated with the streptavidin variant Strep-Tactin. A special crosslinking permits the use for cell isolation and depletion approaches. They were developed for column-based Traceless Affinty Cell Selection (Fab-TACS/Nano-TACS). In this approach, Fab fragments fused to a Twin-Strep-tag (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin Agarose by biotin addition. This way the cells are eluted ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS for exosomes).Alternatively to Fab-Streps/Nano-Streps, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding). This makes Strep-Tactin TACS Agarose columns also suitable for negative cell selection or depletion approaches.

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