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A DNA sequence encoding the glycoprotein G extracellular domain (Asn66-Arg297) of human respiratory syncytial virus A (AMA67228.1) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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A DNA sequence encoding the Human respiratory syncytial virus (RSV) Nucleoprotein (Q6V2F2) (Met1-Leu391) was expressed with an N-terminal polyhistidine tagged AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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