Presentation Supplies

Innovative Research Inc Hmn ATPBndngCstTrnsprtr A4 Kit

Human ATP Binding Cassette Transporter A4 ELISA Kit - This Kit is for the quantitative determination of Human ATP Binding Cassette Transporter A4 in biofluid samples, such as tissue homogenates, cell lysates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests. ABCA4 present in samples reacts with an anti-Human ABCA4 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-ABCA4 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active ABCA4 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human ABCA4. For RUO.