Agglutinins
Vector Laboratories Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 2mL
Bead diameter ranges in size from 45-165 microns. Matrix is stable in solutions at pH 3-11 as well as many organic solvents. Immobilized lectins are prepared using affinity purified lectins
Sigma Aldrich Fine Chemicals Biosciences LIPASE FROM WHEAT GERM,5G
Lipase from wheat germType I, lyophilized powder, 5-15 units/mg solid
Vector Laboratories DuoLuX Substrate, HRP, 200 ml AS-2074
DuoLuX Chemiluminescent and Fluorescent Substrate, Peroxidase (HRP), 200 ml. The substrate can be used for western, southern, northern, or dot blots, or for ELISA applications. This substrate is about 10 times more sensitive than the alkaline phosphatase substrate BCIP/NBT and about 100 times more sensitive than the peroxidase substrate DAB. In addition to its chemiluminescent properties, the reaction product of the DuoLuX Substrate is also fluorescent. Features: Unique formula based on acridan chemistry, Very high sensitivity, Prolonged and intense light emission, Permanent fluorescence. Luminescence: peak emission at 453 nm. Fluorescence: excitation maximum at 405 nm (also excites at 254 nm and 365 nm). Maximum fluorescence emission at 453 nm.
Biotium Wheat Germ Agglutinin, CF405S Conjugate
This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF405S dye has excitation/emission at 404/431 nm.
Vector Laboratories Wheat Germ Agglutinin (WGA) Unconjugated 25 mg
Wheat germ agglutinin (WGA) contains a group of closely related isolectins that bind N-acetylglucosamine (preferentially the dimers and trimers of this sugar) and oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA also interacts with some glycoproteins via sialic acid residues (see succinylated WGA). This unconjugated WGA is used for the purification of insulin receptors and for neuronal tracing and is supplied as a solution preserved with sodium azide. The recommended inhibiting/eluting sugar is chitin hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution.
Vector Laboratories Peanut Agglutinin (PNA) Rhodamine-labeled 5 mg
Peanut agglutinin (PNA) binds the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates and other soluble and membrane-associated glycoproteins and glycolipids. With exceptions, the PNA receptor sequence is normally sialylated, which prevents binding to its receptor oligosaccharide (see Jacalin). Sialic acid not bound to receptor sugars may also inhibit binding, and including calcium in diluents can enhance binding. This rhodamine-labeled PNA features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 575 nm, emission 600 nm). It is supplied as a solution essentially free of unconjugated fluorophores.
Vector Laboratories Peanut agglutinin (PNA) Biotinylated 5 mg
Peanut agglutinin (PNA) binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and other soluble and membrane-associated glycoproteins and glycolipids. With some exceptions, the PNA receptor sequence is normally sialylated, which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid that is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Vector Laboratories Peanut Agglutinin (PNA) Unconjugated 25 mg
Peanut agglutinin (PNA) binds the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates, gangliosides, and other soluble and membrane-associated glycoproteins and glycolipids. With exceptions, the glycan that PNA binds PNA is normally sialylated, which prevents binding. PNA can distinguish between normal and tumor tissues and is used to assess malignancy in transitional mucosa. PNA binding is also used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and measure the levels of lymphoid cell populations in many diseases. PNA has been used for fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers. The inhibiting/eluting sugar is 200 mM galactose.
Biotium Wheat Germ Agglutinin (WGA) Conjugate 1mg
This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm.
Biotium Wheat Germ Agglutinin, CF633 Conjugate
This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF633 dye has excitation/emission at 630/650 nm.