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A protein fucosylation inhibitor; inhibits fucosylation of IgG1 monoclonal antibodies produced by CHO-DG44 cells at 50 µM; reduces intracellular GDP-fucose levels in CHO-DG44 cells; decreases intracellular fucose levels in A. thaliana seedlings at 100 µM; decreases root length in A. thaliana seedlings
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The SeqPlex-i WGA kit allows amplification of small quantities of DNA or from degraded/highly fragmented DNA for direct input onto Illumina(R) next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps Pre-amplification/Library Synthesis Amplification 1 and Amplification 2.Step 1 In the Pre-amplification/Library Synthesis step (using the Library Preparation Reagents) the template DNA is replicated using primers comprised of semi-degenerate 3- ends with universal 5- ends. As polymerization proceeds displaced single strands serve as new templates for additional primer annealing and extension producing random overlapping replicons flanked by a universal primer (5) and primer complement (3) sequence.Step 2 In the Amplified Library Synthesis step (using the Amplification 1 Reagents) products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typically range from 200
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Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
Ulex europaeus agglutinin I (UAE I) binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It is also an established marker for human endothelial cells. This fluorescein-labeled UAE I features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is 50-100 mM L-fucose.
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7-Alkynyl-Fucose is a derivative of fucose used as a high sensitivity research imaging probe in research as well as other applications in cellular biology It has a molecular weight of 356 32 g/mol and is supplied as an amorphous white powder This product is for in vitro research use only This product is not for human or animal use
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