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Agglutinins are biological substances that cause specific cells, or other small particles, to clump or stick together and precipitate out of a solution.
Dolichos biflorus agglutinin (DBA) binds α-linked N-acetylgalactosamine. A glycoprotein consisting of four subunits of approximately equal size and with a molecular weight of about 111 kDa, DBA is used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. It has also been used as a general marker of developing renal collecting ducts. This fluorescein-labeled DBA features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is 200 mM N-acetylgalactosamine.
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A protein fucosylation inhibitor; inhibits fucosylation of IgG1 monoclonal antibodies produced by CHO-DG44 cells at 50 µM; reduces intracellular GDP-fucose levels in CHO-DG44 cells; decreases intracellular fucose levels in A. thaliana seedlings at 100 µM; decreases root length in A. thaliana seedlings
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