Agglutinins
Aat Bioquest iFluor 488-Wheat Germ Agglutinin WGA Conjugate (1 mg)
Wheat germ agglutinin WGA is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. This catalog is for 1 mg.
Aat Bioquest iFluor 555-Wheat Germ Agglutinin WGA Conjugate (1 mg)
Wheat germ agglutinin WGA is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. This catalog is for 1 mg.
Vector Laboratories Succinylated Wheat Germ Agglutinin (WGA), Fluorescein, 5mg
This derivative has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine.
Vector Laboratories Wheat Germ Agglutinin (WGA) Unconjugated 25 mg
Wheat germ agglutinin (WGA) contains a group of closely related isolectins that bind N-acetylglucosamine (preferentially the dimers and trimers of this sugar) and oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA also interacts with some glycoproteins via sialic acid residues (see succinylated WGA). This unconjugated WGA is used for the purification of insulin receptors and for neuronal tracing and is supplied as a solution preserved with sodium azide. The recommended inhibiting/eluting sugar is chitin hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution.
Vector Laboratories Wheat Germ Agglutinin (WGA) Unconjugated 10 mg
Wheat germ agglutinin (WGA) contains a group of closely related isolectins that bind N-acetylglucosamine (preferentially the dimers and trimers of this sugar) and oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA also interacts with some glycoproteins via sialic acid residues (see succinylated WGA). This unconjugated WGA is used for the purification of insulin receptors and for neuronal tracing and is supplied as a solution preserved with sodium azide. The recommended inhibiting/eluting sugar is chitin hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution.
Vector Laboratories Sambucus Nigra Lectin CY3 1 mg L-1010-10
Sambucus Nigra Lectin (SNA, EBL), CY3, 1 mg, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in alpha-2,6 and, to a lesser degree, alpha-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis. Cy3-labeled Sambucus nigra lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. Excitation: 550 nm; Emission: 570 nm; Color: Red.
Vector Laboratories Peanut agglutinin (PNA) Biotinylated 5 mg
Peanut agglutinin (PNA) binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and other soluble and membrane-associated glycoproteins and glycolipids. With some exceptions, the PNA receptor sequence is normally sialylated, which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid that is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Vector Laboratories Wheat Germ Agglutinin (WGA) Agarose Bound 10 ml
Wheat germ agglutinin (WGA) is a group of closely related isolectins. WGA can bind to oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues. This lectin can be used for the purification of insulin receptors and for neuronal tracing. Agarose Bound WGA is prepared using affinity-purified lectins and heat stable, cross-linked 4% agarose beads. The attachment of the lectins through a hydrophilic spacer arm to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins. The recommended inhibiting/eluting sugar is chitin hydrolysate or 500 mM N-acetylglucosamine. Salt and/or acid elution may also be required.
Vector Laboratories Wheat Germ Agglutinin (WGA) Agarose Bound 2 ml
Wheat germ agglutinin (WGA) is a group of closely related isolectins. WGA can bind to oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues. This lectin can be used for the purification of insulin receptors and for neuronal tracing. Agarose Bound WGA is prepared using affinity-purified lectins and heat stable, cross-linked 4% agarose beads. The attachment of the lectins through a hydrophilic spacer arm to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins. The recommended inhibiting/eluting sugar is chitin hydrolysate or 500 mM N-acetylglucosamine. Salt and/or acid elution may also be required.
Vector Laboratories Wheat Germ Agglutinin (WGA), Biotinylated 5 mg
Wheat germ agglutinin (WGA) binds N-acetylglucosamine, preferentially to dimers and trimers of this sugar. It can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA also interacts with some glycoproteins via sialic acid residues (see succinylated WGA). This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Vector Laboratories Succinylated WGA, Biotinylated DL-1067-1
Succinylated Wheat Germ Agglutinin (WGA), Biotinylated, 5 mg. This derivative has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Biotinylated, succinylated WGA has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Vector Laboratories Peanut Agglutinin (PNA) Rhodamine-labeled 5 mg
Peanut agglutinin (PNA) binds the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates and other soluble and membrane-associated glycoproteins and glycolipids. With exceptions, the PNA receptor sequence is normally sialylated, which prevents binding to its receptor oligosaccharide (see Jacalin). Sialic acid not bound to receptor sugars may also inhibit binding, and including calcium in diluents can enhance binding. This rhodamine-labeled PNA features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 575 nm, emission 600 nm). It is supplied as a solution essentially free of unconjugated fluorophores.
Biotium Wheat Germ Agglutinin, CF568 Conjugate
This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 dye has excitation/emission at 562/583 nm.
Biotium Wheat Germ Agglutinin, CF405M Conjugate
This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF405M dye has excitation/emission at 408/452 nm.
Biotium Wheat Germ Agglutinin, CF640R Conjugate
This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF640R dye has excitation/emission at 642/662 nm.
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