Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total PLK1 Detection Kit, 500 Assay Points
HTRF Human Total PLK1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Progranulin Detection Kit, 100,000 Assay Points
HTRF Human Progranulin Detection Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-FGFR3 (Tyr647/648) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-FGFR3 (Tyr647/648) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total PLK1 Detection Kit, 10,000 Assay Points
HTRF Human Total PLK1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Amyloid β 1-42 Detection Kit, 500 Assay Points
HTRF Human Amyloid β 1-42 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human IL-23 / IL23R Binding Kit, 10,000 Assay Points
HTRF Human IL-23 / IL23R Binding Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total SMARCA-2 Detection Kit, Control Lysate
HTRF Human Total SMARCA-2 Detection Kit, Control Lysate
Revvity Health Sciences Inc PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody Cross-Adsorbed
Revvity Health Sciences Inc HTRF Human Total IκKα Detection Kit, 10,000 Assay Points
HTRF Human Total IκKα Detection Kit, 10,000 Assay Points
Molecular Devices Fluorescence Filter, 485 nm, 20 nm Bandwidth
Replacement fluorescence emission filter with holder specified for a 485 nm center wavelength and 20 nm bandwidth. It is intended for use in compatible multi-mode microplate readers to support fluorescence, luminescence, time-resolved fluorescence (TRF), and BRET1 donor emission assays.
- Center wavelength 485 nm.
- Bandwidth 20 nm.
- Designed for fluorescence, luminescence, time-resolved fluorescence, and BRET1 donor emission assays.
- Includes holder for installation in interchangeable filter slots.
- Intended for use with multi-mode microplate readers that accept interchangeable filters.
Revvity Health Sciences Inc HTRF Human Total JAK1 Detection Kit, 500 Assay Points
HTRF Human Total JAK1 Detection Kit, 500 Assay Points
Molecular Devices Multi-Mode Microplate Reader, 6-384 Well, Monochromator
The SpectraMax M5 is a benchtop multi-mode microplate reader designed for absorbance, fluorescence, luminescence, time-resolved fluorescence, and fluorescence polarization assays. It uses scanning monochromators and AutoPMT optimization to extend dynamic range, and integrates with SoftMax Pro software and automation accessories.
- Detection modes: Absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization.
- Wavelength selection: Scanning monochromators with 1 nm increments across the UV-visible spectrum.
- Plate formats: Supports 6-384 well microplates.
- Light source: Xenon flash lamp for excitation.
- Dynamic range: Extended dynamic range via AutoPMT optimization.
- Temperature control: Ambient +2°C to 60°C.
- Automation: Integrates with plate stacker automation for walk-away operation.
- Approximate dimensions: 22 cm x 58 cm x 39 cm.
Molecular Devices Filter Lens, 550 nm, EMax and VMax Compatible
Replacement optical filter lens with a center wavelength of 550 nm for use in microplate readers. Designed as a field-replaceable spare to restore or modify detection for assays that require a 550 nm bandpass. Supplied as a single unit for straightforward replacement.
- Center wavelength 550 nm.
- Compatible with EMax and VMax microplate readers.
- Single replacement filter, sold each.
- Suitable for absorbance and fluorescence measurements requiring a 550 nm bandpass.
Molecular Devices Fluorescence Polarization Filter, Polarized Vertical, 300-750 nm
Molecular Devices fluorescence polarization filter for fluorescence polarization assays across a broad UV-visible range. The filter is supplied in a vertical polarization configuration and is intended for use in filter-based microplate readers that support fluorescence polarization.
- Broad wavelength range: 300-750 nm.
- Vertical polarization orientation.
- Designed for fluorescence polarization measurements.
- Installs in a filter holder or slide for filter-based microplate readers.
- Suitable for quantitative polarization assays.