Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total Raf1 Detection Kit, Control Lysate
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Molecular Devices Fluorescence Polarization Filter, 595 nm, 35 nm BW
A fluorescence polarization emission filter optimized for 595 nm detection with a 35 nm bandwidth and vertical polarization. Designed for fluorescence polarization assays, the filter improves signal selectivity and reduces background when detecting TAMRA-like red fluorophores. It is compatible with filter-based microplate readers.
- Center wavelength 595 nm for red emission detection.
- 35 nm bandwidth to balance sensitivity and selectivity.
- Vertical polarization for fluorescence polarization measurements.
- Suitable for use in filter-based microplate reader filter holders.
- Optimized for TAMRA and similar red fluorophores.
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Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit, 500 Assay Points
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Revvity Health Sciences Inc HTRF Human Total CDK12 Detection Kit, 10,000 Assay Points
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Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Phospho-ERα (Ser167) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Phospho-ERα (Ser167) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total PLK1 Detection Kit, 10,000 Assay Points
HTRF Human Total PLK1 Detection Kit, 10,000 Assay Points
Molecular Devices Absorbance Filter, 600 nm, 8 nm Bandwidth
The Molecular Devices absorbance bandpass filter centered at 600 nm with an 8 nm full width at half maximum (FWHM) bandwidth is a replacement optical filter for compatible microplate readers. It enables narrow-band absorbance measurements for endpoint and kinetic assays and installs as an interchangeable filter slide.
- Enables narrow-band absorbance measurements at 600 nm.
- 8 nm full width at half maximum bandpass for spectral selectivity.
- Designed for installation as an interchangeable filter slide in compatible microplate readers.
- Suitable for quantitative absorbance assays and endpoint or kinetic measurements.
- Replacement spare for instruments that accept interchangeable absorbance filters.
Revvity Health Sciences Inc HTRF Human Total JAK1 Detection Kit, Control Lysate
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Molecular Devices Absorbance Filter, 260 nm, 5.5 nm Bandwidth
The Molecular Devices 260 nm absorbance filter (5.5 nm bandwidth) is an optical bandpass accessory designed for use with compatible FilterMax series multimode microplate readers. It provides a narrow UV transmission centered at 260 nm for precise absorbance measurements of nucleic acids and other UV-absorbing analytes.
- Provides narrow bandpass transmission centered at 260 nm.
- 5.5 nm bandwidth for selective UV absorbance measurements.
- Suitable for nucleic acid quantification and other UV-absorbing samples.
- Designed for easy installation in modular filter holders.
- Improves measurement specificity by reducing out-of-band light.
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