Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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Molecular Devices MOLECULAR DEVICES
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The SpectraDrop High-Throughput Screening (HTS) Kit provides consumables and a microplate adapter to enable automated, low-volume sample measurement using micro-volume microplates. It combines multiple low-volume slide formats and cover slides to support high-throughput nucleic acid and absorbance assays with minimal sample consumption.
- Contains one microplate adapter for automated plate handling.
- Includes five 24-well low-volume bottom slides.
- Includes five 64-well low-volume bottom slides.
- Includes five 2 μL cover slides and five 4 μL cover slides.
- Supports 2 μL and 4 μL sample volumes (approx. 0.5 mm and 1 mm pathlengths).
- Suitable for high-throughput nucleic acid quantitation and absorbance measurements.
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Chemglass Life Sciences CHEMGLASS LIFE SCIENCES LLC
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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This standards kit is suitable for performance verification of a UV Patrol System. It includes three 10 mL ampules of standard solutions. Solution 1 contains 0.1000 mg/mL Caffeine. Solution 2 contains 4.00 μg/mL each of 2-Acetylfuran, Acetanilide, Acetophenone, Propiophenone, Butylparaben, Benzophenone, and Valerophenone. Solution 3 contains 4.000 mg/mL Caffeine.
- Detection method: UV
- Formulation: Liquid
- Storage condition: Room temperature
- System type: LC
- Instrument: PATROL UPLC
- Quantitative: Yes
- Shelf life: 24 Months
- Shipping condition: Ambient
- Volume: 10 mL per ampule
- Application: System performance
- Product type: Standards
- Units per package: 1 pk
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