Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Excitation Filter, 585 nm, 10 nm Bandwidth
This excitation filter provides narrowband fluorescence excitation centered at 585 nm with a 10 nm full width, for use in compatible multi-mode microplate readers.
- Center wavelength 585 nm.
- Bandwidth 10 nm full width.
- Excitation filter type suitable for narrowband fluorescence assays.
- Designed for installation in standard filter slide assemblies.
Revvity Health Sciences Inc HTRF XIAP BIR3 Binding Kit Red Ligand, 1 Vial
HTRF XIAP BIR3 Binding Kit Red Ligand, 1 Vial
Revvity Health Sciences Inc HTRF Human Total ATG14 Detection Kit, 10,000 Assay Points
HTRF Human Total ATG14 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total p21 CiP1 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Total p21 CiP1 Detection Kit, 500 Assay Points
Molecular Devices Multi-Mode Microplate Reader, Five-Mode, HTRF Compatible, 6-384 Wells
The SpectraMax iD5 is a compact, five-mode multi-mode microplate reader designed for flexible bench-top detection of absorbance, fluorescence, luminescence, time-resolved fluorescence, and fluorescence polarization assays. The reader supports optional modules for HTRF and TR-FRET, bottom-read luminescence, and injector-based flash assays, and accepts 6-384 well plates.
- Five built-in detection modes for absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization.
- Expandable detection for HTRF, TR-FRET, BRET, and bottom-read luminescence with optional modules.
- Supports 6-384 well plate formats for endpoint and kinetic assays.
- Xenon flash light source with PMT and photodiode detectors for broad wavelength coverage.
- Temperature control to support assays up to 66°C and multiple shaking modes for assay mixing.
- Robotic compatibility for high-throughput automation workflows.
Revvity Health Sciences Inc HTRF Human Androgen Receptor Detection Kit, Control Lysate
HTRF Human Androgen Receptor Detection Kit, Control Lysate
Molecular Devices Fluorescence Polarization Filter, Polarized Vertical, 300-750 nm
Molecular Devices fluorescence polarization filter for fluorescence polarization assays across a broad UV-visible range. The filter is supplied in a vertical polarization configuration and is intended for use in filter-based microplate readers that support fluorescence polarization.
- Broad wavelength range: 300-750 nm.
- Vertical polarization orientation.
- Designed for fluorescence polarization measurements.
- Installs in a filter holder or slide for filter-based microplate readers.
- Suitable for quantitative polarization assays.
Revvity Health Sciences Inc Saw Tooth (Fine) Generator Probe, 20 x 195 mm
Saw Tooth (Fine) Generator Probe, 20 x 195 mm
Revvity Health Sciences Inc HTRF Human Total CDK7 Detection Kit, 10,000 Assay Points
HTRF Human Total CDK7 Detection Kit, 10,000 Assay Points
Molecular Devices RS-232C Cable, DB-25 Male to DB-9 Female, Straight-Through
This dedicated RS-232C serial cable connects MAXline series microplate readers (EMax, VMax, UVMax, ThermoMax) to a computer RS-232 port. It uses a DB-25 male connector at the instrument end and a DB-9 female connector at the computer end and follows the manufacturer's straight-through RS-232C pin routing for reliable bidirectional communication.
- Connector type: DB-25 male (instrument) to DB-9 female (computer).
- Interface: RS-232C, straight-through wiring.
- Shielding: double-shielded cable recommended for noise reduction.
- Compatibility: MAXline family microplate readers with DB-25 RS-232 instrument port.
- Pin wiring: manufacturer-specified 25-to-9 pin routing for bidirectional communication.
- Cable length: not specified in available documentation.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total TIE2 Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Total TIE2 Detection Kit, 100 Assay Points
Revvity Health Sciences Inc HTRF Human Total DDR1 Detection Kit, 10,000 Assay Points
HTRF Human Total DDR1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit, 10,000 Assay Points
HTRF Human IL-1β High Performance Detection Kit, 10,000 Assay Points
Molecular Devices Fluorescence Polarization Filter, 625 nm, 35 nm, Vertical
Fluorescence polarization emission filter with holder designed for use in compatible SpectraMax microplate readers. The filter provides a 625 nm center wavelength with a 35 nm bandwidth and a vertical polarization axis, optimized for fluorescence polarization assays and red-emitting fluorophores.
- Center wavelength 625 nm.
- Bandwidth 35 nm full width.
- Polarized vertical orientation.
- Includes holder for slide mounting.
- Optimized for fluorescence polarization assays and red-emitting fluorophores.
- Compatible with microplate readers that accept filter slides.