Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF cIAP1 BIR2 Binding Kit, 500 Assay Points
HTRF cIAP1 BIR2 Binding Kit, 500 Assay Points
Alkali Scientific ASI™ Black Confocal Dish 35 x10mm, Easy-Grip, Adhesion Type, Sterile, Treated (Positive-Charged/ Surface Coated), 50/Cs, 22 x 22 x 0.13mm, Hole 3.14mm, PS/Flux
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products:Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy Bottom materials: Glass/FLux (no surface treatment for glass bottom) Coverslip: #1.5 Dish color: Clear/Black Dish size: 35 Ø Hole size: 13 Ø / 20 Ø / 25Ø Non – pyrogenic Non – cytotoxic DNase / RNase – free Human DNA – free
Revvity Health Sciences Inc HTRF Human Total BRD2 Detection Kit, 10,000 Assay Points
HTRF Human Total BRD2 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total HDAC4 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total HDAC4 Detection Kit, 500 Assay Points
Perkin Elmer US LLC Manual Injector Bracket
Magnetic Clip-on Bracket for Manual Injection on FX series LC Instruments.
Revvity Health Sciences Inc HTRF EPIgeneous Binding Domain B Detection Kit, 10,000 Assay Points
HTRF EPIgeneous Binding Domain B Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total MET Detection Kit, 500 Assay Points
HTRF Human Total MET Detection Kit, 500 Assay Points
Molecular Devices Fluorescence Excitation Filter, 535 nm, 25 nm bandwidth
Molecular Devices 535 nm fluorescence excitation filter (MPN 6590-0057) is a bandpass excitation filter centered at 535 nm with a 25 nm bandwidth, intended for installation in compatible microplate reader filter slides to provide selective excitation for fluorescence assays.
- Excitation bandpass centered at 535 nm with a 25 nm bandwidth.
- Optimized for fluorescence assay excitation to improve signal specificity.
- Form factor designed for installation in compatible microplate reader filter slides.
- Intended for use with multi-mode microplate readers that accept filter slides.