Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF cIAP1 BIR3 Binding Kit, 500 Assay Points
HTRF cIAP1 BIR3 Binding Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total CDK5 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total CDK5 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total RBM39 Detection Kit, Control Lysate
HTRF Human & Mouse Total RBM39 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD7 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total BRD7 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit, 96 Assay Points
HTRF Human IL-1β High Performance Detection Kit, 96 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-MET (Thr1234/1235) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-MET (Thr1234/1235) Detection Kit, 50,000 Assay Points
Molecular Devices Absorbance Filter, 280 nm, 12 nm Bandwidth, Slide
Molecular Devices absorbance filter designed for narrow-band UV absorbance measurements centered at 280 nm. The filter is supplied as a slide-mounted optical element for installation in compatible microplate reader filter slots and improves accuracy for deep-UV absorbance assays.
- Center wavelength 280 nm.
- Bandwidth 12 nm.
- Narrow-bandpass optical filter for UV absorbance measurements.
- Slide-mounted form factor for slot installation.
- Optimized for protein and nucleic acid absorbance assays.
Chemglass Life Sciences Dual Chamber Only, Vacuum Trap
Dual Chamber Only, Vacuum Trap. Replacement part for CG42501 dual chamber vacuum trap.
Revvity Health Sciences Inc HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, Control Lysate
HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-SMAD4 (Thr277) Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Phospho-SMAD4 (Thr277) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total H2AX Detection Kit, 500 Assay Points
HTRF Human Total H2AX Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total JAK1 Detection Kit, 10,000 Assay Points
HTRF Human Total JAK1 Detection Kit, 10,000 Assay Points
Molecular Devices Polarization Filter, 700 nm BW 60 nm, Vertical
This fluorescence polarization filter in a holder enables near-infrared fluorescence polarization measurements centered at 700 nm and installs into the filter slot of compatible multi-mode microplate readers to support FP assays.
- 700 nm center wavelength with 60 nm bandwidth.
- Fluorescence polarization optical design for polarization-based measurements.
- Vertical polarization orientation for polarization detection.
- Mounted in a holder for insertion into reader filter slots.
- Intended for fluorescence polarization assays and near-IR detection workflows.
Revvity Health Sciences Inc HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 50,000 Assay Points