Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Electron Microscopy Sciences Nagoette Single Counting Cell 0.5 mm
Counting cells are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting. All complete with case and 1 pair of cover glasses. The depth of the Nageotte counting is 0.5 mm. For Liquids in Poor content.
Revvity Health Sciences Inc HTRF Human XIAP / BIR3 Binding Kit, 10,000 Assay Points
HTRF Human XIAP / BIR3 Binding Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total Estrogen Receptor α Detection Kit, 50,000 Assay Points
HTRF Human Total Estrogen Receptor α Detection Kit, 50,000 Assay Points
Electron Microscopy Sciences Counting Chamber Cover Glass, 25 x 33x0.5mm
For use with counting chambers.
Size: 25 x 33 x 0.5 mm
Revvity Health Sciences Inc HTRF Human IL-23 / IL12R-β1 Binding Kit, 500 Assay Points
HTRF Human IL-23 / IL12R-β1 Binding Kit, 500 Assay Points
Molecular Devices Fluorescence Emission Filter, 550 nm, 10 nm
Emission filter centered at 550 nm with a 10 nm full width at half maximum (FWHM), intended for selective fluorescence detection in multi-mode microplate readers that accept standard filter slides or mounts.
- Center wavelength 550 nm.
- Bandwidth 10 nm (FWHM).
- Emission bandpass design for fluorescence detection.
- Reduces spectral crosstalk and improves signal specificity.
- Suitable for use in multi-mode microplate readers and compatible filter slide systems.
Revvity Health Sciences Inc HTRF Strep-Tactin Eu-Conjugate, 20,000 Assay Points
HTRF Strep-Tactin Eu-Conjugate, 20,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, Control Lysate
HTRF Human Phospho-ATM (Ser1981) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total ULK1 Detection Kit, 50,000 Assay Points
HTRF Human Total ULK1 Detection Kit, 50,000 Assay Points
Molecular Devices Luminescence Filter, Long-pass 610 nm, Emission
The Molecular Devices 610 nm LP luminescence filter (cat. #6590-0117) is a long-pass emission filter optimized for luminescence detection and BRET acceptor measurements. It is intended for use as an acceptor/emission filter in NanoBRET assays and is documented for compatibility with filter-based multi-mode microplate readers such as the SpectraMax iD5.
- Provides a long-pass emission cutoff at 610 nm.
- Optimized for luminescence and BRET acceptor detection.
- Designed for use in filter-based microplate reader optical systems.
- Supports assays requiring acceptor emission separation in NanoBRET workflows.
Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, 500 Assay Points
HTRF Human Phospho-ATM (Ser1981) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total MerTK Detection Kit, 500 Assay Points
HTRF Human Total MerTK Detection Kit, 500 Assay Points