Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Filter Lens, 562 nm, EMax and VMax Compatible
Replacement optical filter lens providing a 562 nm wavelength selection for microplate reader measurements. It is compatible with Molecular Devices EMax and VMax microplate readers and installs in the instrument filter assembly to enable detection at 562 nm.
- Provides 562 nm wavelength selection for optical measurements.
- Compatible with EMax and VMax microplate readers.
- Supports fluorescence and absorbance assays requiring 562 nm detection.
- Replacement lens for instrument filter assemblies.
- Available on request; contact supplier for availability.
Molecular Devices Time-Resolved Emission Filter, 616 nm, 10 nm Bandwidth
This Molecular Devices time-resolved emission filter provides a 616 nm center wavelength with a 10 nm bandwidth for time-resolved fluorescence (TRF) and TR-FRET detection in multi-mode microplate readers. It is intended to isolate emission bands and improve signal selectivity in time-resolved assays.
- Optimized for time-resolved fluorescence and TR-FRET assays.
- 616 nm center wavelength, 10 nm bandwidth.
- Emission filter configured for use in microplate reader filter holders.
- Improves emission selectivity and reduces background signal.
- Referenced in instrument application notes for optimized TR-FRET settings.
Revvity Health Sciences Inc HTRF Human Phospho-FGFR3 (Tyr647/648) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-FGFR3 (Tyr647/648) Detection Kit, 50,000 Assay Points
Molecular Devices Fluorescence Filter Kit, EM 660 nm, 40 nm
The SpectraMax Fluorescence Intensity Filter Kit delivers five exchangeable optical filters for fluorescence, luminescence, and time-resolved fluorescence (TRF) assays on compatible multi-mode microplate readers. The kit includes an emission 660 nm filter with a 40 nm bandwidth plus additional excitation and emission filters to support common assays.
- Includes five exchangeable filters covering common fluorescence wavelengths from ~400-700 nm.
- Contains an emission 660 nm filter with a 40 nm full bandwidth for near-IR fluorescence detection.
- Supports fluorescence intensity, luminescence, and time-resolved fluorescence assay modes.
- Allows quick filter exchange to adapt to different assay requirements.
- Designed for use with compatible multi-mode microplate readers to expand detection flexibility.
Revvity Health Sciences Inc HTRF Human Total FGFR1 Detection Kit, 50,000 Assay Points
HTRF Human Total FGFR1 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Mutant HTT Detection Kit, 100,000 Assay Points
HTRF Human & Mouse Mutant HTT Detection Kit, 100,000 Assay Points
Electron Microscopy Sciences USAF Test Chart R75P Positive Image
USAF resolution charts are recognized the world over as a universal standard for testing the vertical and horizontal resolution of imaging systems. Each element on the chart comprises three vertical bars and three horizontal bars, and the detail on these slides is as fine as 0.78microns (644 line-pairs per mm). The resolution of the imaging system is normally specified as the Group and Element of the finest bars that can be clearly defined. Sub Micron smallest feature size - bar width 0.00078mm [0.78 um].
Type: Positive Image
Use: Groups 0/1 to 9/3
Perkin Elmer US LLC Complete Capillary Injector Add On Kit for 240 V AutoSystem
Complete Capillary Injector Add On Kit for 240 V AutoSystem
Revvity Health Sciences Inc HTRF Anti-VHH Tb-Antibody, 5,000 Assay Points
HTRF Anti-VHH Tb-Antibody, 5,000 Assay Points