Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total PR Detection Kit, 10,000 Assay Points
HTRF Human Total PR Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD9 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total BRD9 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit, 100 Assay Points
Molecular Devices Fluorescence Filter, 400 nm, 30 nm BW, Holder
This fluorescence filter with holder provides an optical band centered at 400 nm with a 30 nm bandwidth for use in microplate-based fluorescence assays, luminescence, and time-resolved fluorescence detection. It serves as an accessory optical element for multimode microplate readers.
- 400 nm center wavelength, 30 nm bandwidth
- Designed for fluorescence, luminescence, and time-resolved fluorescence detection
- Includes mounting holder for installation in standard filter slots
- Intended for use with multimode microplate readers and plate-based assays
Revvity Health Sciences Inc HTRF Human IL-23 / IL12R-β1 Binding Kit, 10,000 Assay Points
HTRF Human IL-23 / IL12R-β1 Binding Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF CD64 Cellular Binding Kit, 1,000 Assay Points
HTRF CD64 Cellular Binding Kit, 1,000 Assay Points
Revvity Health Sciences Inc HTRF cIAP1 BIR3 Binding Kit - Standard
HTRF cIAP1 BIR3 Binding Kit - Standard
Enterprise Technology Solutions Customizable TOC Ready Index M
Customizable TOC Ready Index Multicolor Tab Dividers Uncollated 8-Tab 1 to 8 11 x 8.5 White 24 Sets
Revvity Health Sciences Inc HTRF AAV3B Detection Kit, 500 Assay Points
HTRF AAV3B Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human CCL22 Detection Kit Standard
HTRF Human CCL22 Detection Kit Standard
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human Phospho-ErbB3 (HER3) (Tyr1289 & Total) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Multiplex Human Phospho-ErbB3 (HER3) (Tyr1289 & Total) Detection Kit, Control Lysate
Molecular Devices Fluorescence Filter, 625 nm, 35 nm BW
Replacement fluorescence emission filter supplied with a mounting holder for use in compatible Molecular Devices microplate readers. The filter provides a red-region emission band centered at 625 nm with a 35 nm full bandwidth, suitable for fluorescence intensity and time-resolved fluorescence assays.
- Center wavelength 625 nm.
- Bandwidth 35 nm full width.
- Includes mounting holder for slide insertion.
- Optimized for red-emission fluorescence detection.
- Intended for use in microplate readers that accept plug-in filters.