Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Fluorescence Polarization Filter, 590 nm, 20 nm, Horizontal
This fluorescence polarization filter isolates emission at 590 nm with a 20 nm bandpass and is supplied with a holder for installation in compatible microplate readers, including SpectraMax models. It is intended for fluorescence polarization assays where precise emission isolation and polarized detection are required.
- Center wavelength 590 nm.
- Bandwidth 20 nm.
- Polarized orientation horizontal.
- Includes holder for instrument installation.
- Designed for fluorescence polarization assays.
- Compatible with polarization detection modules in microplate readers.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Phospho-RNA polymerase II (Ser5) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Phospho-RNA polymerase II (Ser5) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Total p300 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total p300 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, Control Lysate
HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total CDK1 Detection Kit, 50,000 Assay Points
HTRF Human Total CDK1 Detection Kit, 50,000 Assay Points
Molecular Devices Fluorescence Polarization Filter, 635 nm, 35 nm BW
Molecular Devices fluorescence polarization filter specified for a 635 nm center wavelength with a 35 nm bandwidth. It is designed for fluorescence polarization assays in microplate and ELISA readers and is supplied with a holder for insertion into compatible filter modules.
- Suitable for fluorescence polarization measurements.
- 635 nm center wavelength, 35 nm bandwidth.
- Polarized horizontal orientation.
- Includes holder for filter module insertion.
- Intended for microplate and ELISA reader applications.
Revvity Health Sciences Inc HTRF AAV9 Detection Kit, 10,000 Assay Points
HTRF AAV9 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total Cyclin D1 Detection Kit, Control Lysate
HTRF Human & Mouse Total Cyclin D1 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human XIAP / BIR3 Binding Kit, 100,000 Assay Points
HTRF Human XIAP / BIR3 Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total PR Detection Kit, 500 Assay Points
HTRF Human Total PR Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human XIAP / BIR3 Binding Kit Standard
HTRF Human XIAP / BIR3 Binding Kit Standard
Molecular Devices Fluorescence Polarization Filters, 535 nm, Polarized
Molecular Devices fluorescence polarization filter set designed for fluorescence polarization (FP) assays on compatible SpectraMax and FlexStation multi-mode microplate readers. The set contains polarized filters in vertical and horizontal orientations centered at 535 nm with a 25 nm bandwidth to support accurate FP measurements.
- Enables fluorescence polarization measurements.
- Includes vertical and horizontal polarized filters.
- Center wavelength 535 nm, bandwidth 25 nm.
- Includes two filters for polarization detection.
- Designed for compatible multi-mode microplate readers.