Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total ULK1 Detection Kit, 50,000 Assay Points
HTRF Human Total ULK1 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total SMARCA-4 Detection Kit, 500 Assay Points
HTRF Human Total SMARCA-4 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, 500 Assay Points
HTRF Human Phospho-ATM (Ser1981) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total FAK Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total FAK Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human CCL22 Detection Kit, 100,000 Assay Points
HTRF Human CCL22 Detection Kit, 100,000 Assay Points
Molecular Devices Fluorescence Filter, 700 nm, 60 nm Bandwidth, Holder
Molecular Devices fluorescence emission filter with holder (MPN 6590-0114) provides selective far-red detection at a 700 nm center wavelength with a 60 nm optical bandwidth. It is sold as a replaceable accessory for compatible multi-mode microplate readers and mounts in the instrument's filter holder for reliable signal selection.
- Center wavelength: 700 nm.
- Bandwidth: 60 nm.
- Filter type: emission filter for far-red fluorescence.
- Includes holder for instrument mounting.
- Typical application: far-red fluorescence assays on compatible readers.
Molecular Devices Excitation Filter, 485 nm, 20 nm Bandwidth
Excitation filter for fluorescence microplate assays centered at 485 nm with a 20 nm bandwidth. The filter is designed for installation in filter slide holders and is used to selectively excite blue-range fluorophores in fluorescence intensity and ELISA workflows.
- Precise 485 nm center wavelength for blue-excited fluorophores.
- 20 nm bandwidth for selective excitation and reduced background signal.
- Designed for installation in filter slide holders used by multi-mode microplate readers.
- Suitable for fluorescence intensity assays and ELISA workflows.
- Replaceable optical element for routine maintenance and assay optimization.
Molecular Devices Fluorescence Excitation Filter, 550 nm, 10 nm
A narrow-band fluorescence excitation filter centered at 550 nm with a 10 nm full width at half maximum (FWHM), designed for microplate fluorescence assays to isolate excitation light and improve signal specificity.
- Provides narrow-band excitation centered at 550 nm.
- 10 nm full width at half maximum for selective excitation.
- Optimized for microplate fluorescence measurements.
- Reduces background and improves signal-to-noise ratio.
- Designed for use in compatible microplate fluorescence readers.
Molecular Devices Fluorescence Filter, 447 nm, 60 nm, With Holder
Fluorescence donor emission filter for SpectraMax iD5 and iD5e multi-mode microplate readers, centered at 447 nm with a 60 nm bandwidth, supplied with a holder for mounting and optical alignment.
- Isolates emission centered at 447 nm with a 60 nm bandwidth.
- Includes holder for secure mounting in the instrument filter module.
- Compatible with fluorescence and NanoBRET assays on multi-mode microplate readers.
- Improves signal separation for donor emission detection and assay sensitivity.
- Designed for easy installation and reliable optical alignment.
Molecular Devices Emission Filter, Time-Resolved, 665 nm, 10 nm
Molecular Devices time-resolved acceptor emission filter supplied as a filter slide with holder. It provides a 665 nm center wavelength with a 10 nm bandwidth for sensitive acceptor emission detection in time-resolved fluorescence assays and is designed for use in compatible multi-mode microplate readers.
- Center wavelength 665 nm.
- Bandwidth 10 nm.
- Filter type time-resolved acceptor emission.
- Form factor filter slide with holder.
- Intended applications TR-FRET and HTRF assays.
- Compatible with multi-mode microplate readers that accept filter slides.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Phospho-IκBα (Ser32/36) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Phospho-IκBα (Ser32/36) Detection Kit, 500 Assay Points