Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Androgen Receptor Full Length, Control Lysate
HTRF Human Androgen Receptor Full Length, Control Lysate
Revvity Health Sciences Inc HTRF PROTAC Binding Buffer #1, 200 mL
HTRF PROTAC Binding Buffer #1, 200 mL
Revvity Health Sciences Inc HTRF Human IL-2 High Performance Detection Kit, 10,000 Assay Points
HTRF Human IL-2 High Performance Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Strep-Tactin Eu-Conjugate, 20,000 Assay Points
HTRF Strep-Tactin Eu-Conjugate, 20,000 Assay Points
Molecular Devices Time Resolved Filter, 642 nm, 10 nm Bandwidth
Manufactured by Molecular Devices, this time-resolved interference filter provides a narrow optical band centered at 642 nm with a 10 nm full width at half maximum (FWHM) for improved signal specificity in time-resolved fluorescence assays. It is intended as a plug-in accessory for compatible multi-mode microplate readers to enable sensitive TRF measurements.
- Center wavelength: 642 nm.
- Bandwidth: 10 nm FWHM.
- Filter type: time-resolved fluorescence interference filter.
- Optimized for time-resolved fluorescence assays to reduce background signal.
- Designed as a plug-in accessory for compatible multi-mode microplate readers.
Molecular Devices Absorbance Filter, 420 nm, 8 nm bandwidth, FilterMax
Molecular Devices FilterMax absorbance filter designed for fixed-wavelength absorbance assays. The filter provides narrowband transmission centered at 420 nm with approximately 8 nm full width at half maximum and is supplied as an accessory for FilterMax microplate readers.
- Provides narrowband transmission centered at 420 nm.
- Approximately 8 nm full width at half maximum for high wavelength specificity.
- Optimized for absorbance assays on compatible multimode readers.
- Supplied as a replaceable accessory for FilterMax F3 and F5 series readers.
Molecular Devices Absorbance Filter, 450 nm, 8 nm, FilterMax Compatible
Replaceable narrow-band absorbance (photometric) filter designed for use in FilterMax multimode microplate readers. The filter is centered at 450 nm and has an approximate 8 nm bandwidth, providing a specific narrow bandpass for fixed-wavelength absorbance measurements in microplates.
- Center wavelength: 450 nm.
- Bandwidth: 8 nm (approximate).
- Designed for fixed-wavelength absorbance assays.
- Installs in the instrument filter wheel for modular wavelength selection.
- Narrow-bandpass optical filter for consistent photometric readings.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total Raf1 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Total Raf1 Detection Kit, Control Lysate
Molecular Devices Fluorescence Polarization Filter, 595 nm, 35 nm BW
A fluorescence polarization emission filter optimized for 595 nm detection with a 35 nm bandwidth and vertical polarization. Designed for fluorescence polarization assays, the filter improves signal selectivity and reduces background when detecting TAMRA-like red fluorophores. It is compatible with filter-based microplate readers.
- Center wavelength 595 nm for red emission detection.
- 35 nm bandwidth to balance sensitivity and selectivity.
- Vertical polarization for fluorescence polarization measurements.
- Suitable for use in filter-based microplate reader filter holders.
- Optimized for TAMRA and similar red fluorophores.
Revvity Health Sciences Inc HTRF Human Total CDK12 Detection Kit, 10,000 Assay Points
HTRF Human Total CDK12 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total JAK1 Detection Kit, Control Lysate
HTRF Human Total JAK1 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Total MSH3 Detection Kit, Control Lysate
HTRF Total MSH3 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total KRAS Detection Kit, 50,000 Assay Points
HTRF Human Total KRAS Detection Kit, 50,000 Assay Points
Molecular Devices Fluorescence Polarization Filter, 700 nm, Excitation
Molecular Devices 700 nm fluorescence polarization filter designed for fluorescence polarization (FP) microplate assays and near-infrared fluorescence detection. It provides excitation and detection at 700 nm for compatible multi-mode microplate readers.
- Provides excitation and detection centered at 700 nm for fluorescence polarization assays.
- Optimized for fluorescence polarization measurements in microplate formats.
- Enables near-infrared fluorescence detection to reduce background and improve signal-to-noise.
- Designed for easy installation in compatible filter holders and cartridges.
- Suitable for use with multi-mode microplate readers that support user-installable filters.