Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Perkin Elmer US LLC Split/Splitless Injector Starter Kit for Manual Gas Control
Includes the following pieces: (5) Ultra Inert Splitless Liners with Wool 4 mm I.D. (09200696) (5) Ultra Deactivated Split/Splitless Liners with Glass Wool, 4 mm I.D. (09200624) (10) Silicone O-Rings (09200714) (50) Green Injection Port Septa (N6621028) (10) 0.4 mm (09200685), (10) 0.5 mm (09200686) and (10) 0.8 mm Graphite/Vespel Ferrules (09200687) (2) Gold Seal Washers (N6551043) (1) Gold Seal Nut (N6552080) (1) Capillary Injector Nut (N6552084) (1) Ceramic Column Cutter (N9301376)
Revvity Health Sciences Inc HTRF Human & Mouse Total HDAC4 Detection Kit, Control Lysate
HTRF Human & Mouse Total HDAC4 Detection Kit, Control Lysate
Electron Microscopy Sciences USAF Test Chart R75N Negative Image
USAF resolution charts are recognized the world over as a universal standard for testing the vertical and horizontal resolution of imaging systems. Each element on the chart comprises three vertical bars and three horizontal bars, and the detail on these slides is as fine as 0.78microns (644 line-pairs per mm). The resolution of the imaging system is normally specified as the Group and Element of the finest bars that can be clearly defined. Sub Micron smallest feature size - bar width 0.00078mm [0.78 um].
Type: Negative Image
Use: Groups 0/1 to 9/3
Revvity Health Sciences Inc HTRF Human Total VEGFR2 Detection Kit, 500 Assay Points
HTRF Human Total VEGFR2 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Anti histone H3K4 Me2-EuK mAb conjugate, 10,000 Assay Points
HTRF Anti histone H3K4 Me2-EuK mAb conjugate, 10,000 Assay Points
Molecular Devices Photometric Filter, 405 nm, 10 nm Bandwidth
AD 340 photometric absorbance filter for narrowband absorbance measurements at 405 nm. Designed as a replacement or accessory optical filter for absorbance channels in microplate reader assays, it provides a focused transmission band for reliable endpoint and kinetic measurements.
- Center wavelength: 405 nm.
- Bandwidth: 10 nm.
- Measurement type: Absorbance (photometric).
- Typical applications: Endpoint and kinetic microplate assays.
- Form factor: Optical bandpass photometric filter.
Revvity Health Sciences Inc HTRF CD16a (FcgRIIIa, V158) Cellular Binding Kit, 1,000 Assay Points
HTRF CD16a (FcgRIIIa, V158) Cellular Binding Kit, 1,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total CDK5 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total CDK5 Detection Kit, 500 Assay Points
Molecular Devices Absorbance Filter, 280 nm, 5.5 nm Bandwidth
Molecular Devices 280 nm absorbance filter designed as a replacement slide-style optical filter for select multimode microplate readers. The narrow 5.5 nm bandwidth enables precise absorbance measurements at 280 nm for protein quantitation and other absorbance-based assays.
- Provides narrowband absorbance detection at 280 nm with a 5.5 nm bandwidth.
- Optimized for protein quantitation and small-volume absorbance assays.
- Slide-style form factor suitable for insertion into accessory filter slots.
- Low stray light and high transmission within the passband.
- Intended as a replacement optical filter to restore instrument performance.
Revvity Health Sciences Inc HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, Control Lysate
HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit, 100,000 Assay Points
HTRF Human IL-1β High Performance Detection Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Protein Disaggregation Detection Kit, 100 samples
HTRF Protein Disaggregation Detection Kit, 100 samples
Molecular Devices Filter Lens, 600 nm, Microplate compatible, Replacement
Replacement filter lens for microplate and ELISA readers. This optical component provides centered transmission at 600 nm and is sold as a spare replacement for compatible plate readers including EMax, VMax, UVMax, and ThermoMax models.
- Provides centered transmission at 600 nm for absorbance measurements.
- Designed for use in microplate and ELISA reader detection optics.
- Supplied as a single replacement lens for instrument maintenance.
- Helps maintain assay accuracy for absorbance-based protocols.