Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Sigma Aldrich Fine Chemicals Biosciences TranscreenerR ADP2 TR fRET
The Transcreener(R) ADP2 TR-fRET Red Assay is a competitive immunoassay for ADP with a far-red time-resolved foumlrster-resonance-energy-transfer (TR-fRET) readout. Because it is highly selective for ADP the assay can be used with any enzyme that converts ATP to ADP regardless of what other substrates are used. Examples of enzymes include protein lipid and carbohydrate kinases ATPases DNA helicases carboxylases and glutamine synthetase. The Transcreener(R) assay is designed specifically for high-throughput screening (HTS) with a single addition mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener(R) HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. The Transcreener(R) ADP2 TR-fRET Red Assay provides the following benefitsbullnbsp Accommodates ATP concentra
Glas Col LLC Control
Proportional power controller, 12A, 120V for use with 3 place multi position combo mantles.
Electron Microscopy Sciences Pipette Tip Universal, 5 - 200 mcL
Pipette tips are low binding to ensure accuracy and precision. The low binding surface is the result of a manufacturing process that binds a perfluorooctyl polymer to polypropylene resin–resulting in an exposed trifluoromethyl surface. This surface reduces the surface energy of liquid and repels any substance so sample retention and binding are minimized.
Not detectable/Non-reactive.
Extracted only by a fluorinated solvent.
Autoclaving will not compromise performance.
Available in convenient hinged racked package.
Tips and racks are fully autoclavable. Natural tips feature reference marks at 10 ul, 50 ul, and 100 u. Low binding surface.
Range: 5 - 200 µl
Electron Microscopy Sciences Nagoette Single Counting Cell 0.5 mm
Counting cells are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting. All complete with case and 1 pair of cover glasses. The depth of the Nageotte counting is 0.5 mm. For Liquids in Poor content.
Molecular Devices Time-Resolved Emission Filter, 616 nm, 10 nm Bandwidth
This Molecular Devices time-resolved emission filter provides a 616 nm center wavelength with a 10 nm bandwidth for time-resolved fluorescence (TRF) and TR-FRET detection in multi-mode microplate readers. It is intended to isolate emission bands and improve signal selectivity in time-resolved assays.
- Optimized for time-resolved fluorescence and TR-FRET assays.
- 616 nm center wavelength, 10 nm bandwidth.
- Emission filter configured for use in microplate reader filter holders.
- Improves emission selectivity and reduces background signal.
- Referenced in instrument application notes for optimized TR-FRET settings.
Alkali Scientific ASI™ Black Confocal Dish 35 x10mm, Easy-Grip, Adhesion Type, Sterile, Treated (Positive-Charged/ Surface Coated), 50/Cs, 22 x 22 x 0.13mm, Hole 1.33mm, PS/Flux
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products:Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy Bottom materials: Glass/FLux (no surface treatment for glass bottom) Coverslip: #1.5 Dish color: Clear/Black Dish size: 35 Ø Hole size: 13 Ø / 20 Ø / 25Ø Non – pyrogenic Non – cytotoxic DNase / RNase – free Human DNA – free
Alkali Scientific ASI™ Black Confocal Dish 35 x10mm, Easy-Grip, Adhesion Type, Sterile, Non-Treated (Non-Charged/ Hydrophobic), 100/Cs, 22 x 22 x 0.13mm, Hole 1.33mm, PS/Glass
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products:Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy Bottom materials: Glass/FLux (no surface treatment for glass bottom) Coverslip: #1.5 Dish color: Clear/Black Dish size: 35 Ø Hole size: 13 Ø / 20 Ø / 25Ø Non – pyrogenic Non – cytotoxic DNase / RNase – free Human DNA – free
Revvity Health Sciences Inc HTRF Human IL-12/IL-12Rβ1 Binding Kit, 500 Assay Points
HTRF Human IL-12/IL-12Rβ1 Binding Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total TYK2 Detection Kit, 500 Assay Points
HTRF Human Total TYK2 Detection Kit, 500 Assay Points
Electron Microscopy Sciences S10 Stage Counting Grid 0.05 mm Square
Stage graticules are used for the routine calibration of eyepiece patterns, particularly when alternating between objectives on one microscope or when using the same graticule in different microscopes. Their robust construction makes them ideal for student use and for instructional purposes. The scale or grid is centered on a glass disc mounted in a black anodized aluminum slide 75 mm x 24 mm x 2 mm thick. Transmitted light stage slide with 10 x 10 grid of 0.05mm squares.