Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF PROTAC Binding Buffer #1, 200 mL
HTRF PROTAC Binding Buffer #1, 200 mL
THERMO SCI LSG 3P Universal Tray including 96- 384- and 6-48-well plate adapters for plate without lid
Universal Tray including 96- 384- and 6-48-well plate adapters for plate without lid
Revvity Health Sciences Inc HTRF Human Total IκKα Detection Kit, 50,000 Assay Points
HTRF Human Total IκKα Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total ATF4 Detection Kit, 10,000 Assay Points
HTRF Human Total ATF4 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Phospho-PLC-γ1 (Tyr1253) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Phospho-PLC-γ1 (Tyr1253) Detection Kit, Control Lysate
Dojindo Molecular Technologies Inc ATP Assay Kit-Luminescence (50 tests) is used to determine intracellular levels of ATP by luciferase luminescence assay. High-sensitivity detection can be performed with stable luminescence. 50 tests
Adenosine triphosphate (ATP) is an energy source for living cells and is synthesized by both glycolysis and mitochondrial oxidative phosphorylation. Decreased ATP levels are associated with cancer, aging, neurodegenerative diseases, and mitochondrial diseases. The ATP Assay Kit-Luminescence enables the quantitation of intracellular ATP by luciferase luminescence assay. This kit can be applied for microplate assay. Without a complicated process including medium removal and cell washing, the ATP Assay Kit-Luminescence can generate a stable luminescent signal with a half-life of greater than 3 hours by simply mixing the kit components and adding the reagent. This kit contains an ATP Standard, which provides highly reproducible data without measuring unstable ATP. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
Revvity Health Sciences Inc HTRF Human and mouse Total STAT5 Detection Kit, 10,000 Assay Points
HTRF Human and mouse Total STAT5 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total H2AX Detection Kit, 10,000 Assay Points
HTRF Human Total H2AX Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Androgen Receptor Full Length, Control Lysate
HTRF Human Androgen Receptor Full Length, Control Lysate
Revvity Health Sciences Inc HTRF Human IL-2 High Performance Detection Kit, 10,000 Assay Points
HTRF Human IL-2 High Performance Detection Kit, 10,000 Assay Points
Molecular Devices Absorbance Filter, 420 nm, 8 nm bandwidth, FilterMax
Molecular Devices FilterMax absorbance filter designed for fixed-wavelength absorbance assays. The filter provides narrowband transmission centered at 420 nm with approximately 8 nm full width at half maximum and is supplied as an accessory for FilterMax microplate readers.
- Provides narrowband transmission centered at 420 nm.
- Approximately 8 nm full width at half maximum for high wavelength specificity.
- Optimized for absorbance assays on compatible multimode readers.
- Supplied as a replaceable accessory for FilterMax F3 and F5 series readers.
Molecular Devices Absorbance Filter, 450 nm, 8 nm, FilterMax Compatible
Replaceable narrow-band absorbance (photometric) filter designed for use in FilterMax multimode microplate readers. The filter is centered at 450 nm and has an approximate 8 nm bandwidth, providing a specific narrow bandpass for fixed-wavelength absorbance measurements in microplates.
- Center wavelength: 450 nm.
- Bandwidth: 8 nm (approximate).
- Designed for fixed-wavelength absorbance assays.
- Installs in the instrument filter wheel for modular wavelength selection.
- Narrow-bandpass optical filter for consistent photometric readings.
Enterprise Technology Solutions WetTask Customizable Wet Wipin
WetTask Customizable Wet Wiping System Bucket White/Blue 4/Carton
Revvity Health Sciences Inc HTRF Phospho-Total protein lysis buffer 6, 130 mL
HTRF Phospho-Total protein lysis buffer 6, 130 mL