Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Ace Glass, Inc. Adapter, 0-10 Hi-Vac valve, no.15 O-ring joint to #15Ace-Thred, No-Air
ADAPTER VAC 0-10VLV NO15-NO15
Electron Microscopy Sciences MultiLap™ Fish Otolith Polishing Tool 6-Position Alignment Fixture
For use with Model 195 - 6 Position MultiLap (50168-95).
Electron Microscopy Sciences Counting Chamber Cover Glass, 25 x 33x0.5mm
For use with counting chambers.
Size: 25 x 33 x 0.5 mm
Molecular Devices Time Resolved Filter, 642 nm, 10 nm Bandwidth
Manufactured by Molecular Devices, this time-resolved interference filter provides a narrow optical band centered at 642 nm with a 10 nm full width at half maximum (FWHM) for improved signal specificity in time-resolved fluorescence assays. It is intended as a plug-in accessory for compatible multi-mode microplate readers to enable sensitive TRF measurements.
- Center wavelength: 642 nm.
- Bandwidth: 10 nm FWHM.
- Filter type: time-resolved fluorescence interference filter.
- Optimized for time-resolved fluorescence assays to reduce background signal.
- Designed as a plug-in accessory for compatible multi-mode microplate readers.
Molecular Devices Luminescence Filter, Long-pass 610 nm, Emission
The Molecular Devices 610 nm LP luminescence filter (cat. #6590-0117) is a long-pass emission filter optimized for luminescence detection and BRET acceptor measurements. It is intended for use as an acceptor/emission filter in NanoBRET assays and is documented for compatibility with filter-based multi-mode microplate readers such as the SpectraMax iD5.
- Provides a long-pass emission cutoff at 610 nm.
- Optimized for luminescence and BRET acceptor detection.
- Designed for use in filter-based microplate reader optical systems.
- Supports assays requiring acceptor emission separation in NanoBRET workflows.
Molecular Devices Multi-Mode Microplate Reader, Integrated Pipettor, Dual Monochromators, 6-384 Well
The FlexStation 3 is an integrated benchtop microplate reader that combines multi-mode detection with programmable liquid handling to automate biochemical and cell-based assays and support kinetic measurements.
- Automated 8- or 16-channel pipettor for reagent transfer.
- Measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence.
- Supports 6-384 well plate formats.
- Dual monochromators with 1-nm wavelength selection.
- Top and bottom reading capability with PMT and silicon photodiode detectors.
- SoftMax Pro compatible acquisition and analysis software.
- Compact benchtop footprint, approximately 48 x 58 x 41 cm.
Molecular Devices Filter Lens, 490 nm, Fluorescence, Microplate Reader
The Molecular Devices 490 nm filter lens is an optical filter designed for fluorescence measurements in microplate readers. It transmits light centered at 490 nm to isolate the desired fluorescence band for excitation or emission measurements.
- Center wavelength 490 nm.
- Optical filter lens for fluorescence excitation or emission measurements.
- Designed for use with compatible multi-mode microplate readers.
- Helps reduce background by blocking out-of-band wavelengths.
- Intended as a replacement or spare filter for routine maintenance and assay optimization.
Molecular Devices Emission Filter Lens, 595 nm, Microplate Reader Compatible
The Molecular Devices 595 nm filter lens is an emission optical filter designed for fluorescence detection in compatible microplate readers. It provides selective transmission around 595 nm for assays using red-emitting fluorophores and can be used as a replacement or accessory for reader filter wheels.
- Optimized for emission at approximately 595 nm.
- Suitable for fluorescence assays using Cy3/TRITC and related fluorophores.
- Intended as a replacement or accessory for compatible microplate reader filter wheels.
- Supplied as a single unit for service or maintenance use.
Molecular Devices SpectraDrop Cover Slides, 4 μL, 1.0 mm Path
Molecular Devices SpectraDrop Micro-Volume cover slide refills provide replacement cover slides for low-volume absorbance and fluorescence measurements. Each pack contains three 4 μL cover slides with a 1.0 mm path length, designed to restore and extend micro-volume workflows while minimizing sample consumption.
- Pack contains three 4 μL cover slides.
- Path length 1.0 mm.
- Enables measurements with sample volumes as low as 2 μL.
- Designed for micro-volume absorbance and fluorescence measurements.
- Helps reduce sample consumption and increase throughput.
Molecular Devices Fluorescence Polarization Filter, 625 nm, 35 nm, Vertical
Fluorescence polarization emission filter with holder designed for use in compatible SpectraMax microplate readers. The filter provides a 625 nm center wavelength with a 35 nm bandwidth and a vertical polarization axis, optimized for fluorescence polarization assays and red-emitting fluorophores.
- Center wavelength 625 nm.
- Bandwidth 35 nm full width.
- Polarized vertical orientation.
- Includes holder for slide mounting.
- Optimized for fluorescence polarization assays and red-emitting fluorophores.
- Compatible with microplate readers that accept filter slides.
Molecular Devices Fluorescence Filter, 485 nm, 20 nm Bandwidth
Replacement fluorescence emission filter with holder specified for a 485 nm center wavelength and 20 nm bandwidth. It is intended for use in compatible multi-mode microplate readers to support fluorescence, luminescence, time-resolved fluorescence (TRF), and BRET1 donor emission assays.
- Center wavelength 485 nm.
- Bandwidth 20 nm.
- Designed for fluorescence, luminescence, time-resolved fluorescence, and BRET1 donor emission assays.
- Includes holder for installation in interchangeable filter slots.
- Intended for use with multi-mode microplate readers that accept interchangeable filters.
Molecular Devices Absorbance Filter, 420 nm, 8 nm bandwidth, FilterMax
Molecular Devices FilterMax absorbance filter designed for fixed-wavelength absorbance assays. The filter provides narrowband transmission centered at 420 nm with approximately 8 nm full width at half maximum and is supplied as an accessory for FilterMax microplate readers.
- Provides narrowband transmission centered at 420 nm.
- Approximately 8 nm full width at half maximum for high wavelength specificity.
- Optimized for absorbance assays on compatible multimode readers.
- Supplied as a replaceable accessory for FilterMax F3 and F5 series readers.
Alkali Scientific SmartReader™ 96 Microplate Absorbance Reader, for 96 Well Plates, 115V QTY 1
The Accuris SmartReader 96 is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 well plates. The instrument’s 7 inch touch screen, intuitive software and graphical interface makes it easy to use as a standalone instrument (a separate computer and additional software is not required).