Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Time-Resolved Filter, 350 nm, 60 nm Bandwidth
This time-resolved excitation filter selects short-wavelength excitation light centered at 350 nm with a 60 nm full width, for use in time-resolved fluorescence (TRF) assays on compatible filter-based microplate readers. It is intended for applications that require narrow-band excitation around 350 nm for TRF measurements.
- Time-resolved excitation filter.
- Center wavelength 350 nm.
- Bandwidth 60 nm (full width).
- Optimized for time-resolved fluorescence assays.
- Compatible with SpectraMax iD5 filter module and similar readers.
- Fits standard filter holder assemblies in compatible readers.
Revvity Health Sciences Inc HTRF Human Total EGFR DEL19 Detection Kit, 50,000 Assay Points
HTRF Human Total EGFR DEL19 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total JAK2 Detection Kit, 50,000 Assay Points
HTRF Human Total JAK2 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total PR Detection Kit, 10,000 Assay Points
HTRF Human Total PR Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD9 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total BRD9 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total ATM Detection Kit, Control Lysate
HTRF Human Total ATM Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total PINK3 Detection Kit, 50,000 Assay Points
HTRF Human Total PINK3 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total FLT3 Detection Kit, Control Lysate
HTRF Human Total FLT3 Detection Kit, Control Lysate
Molecular Devices Fluorescence Filter, Holder, 360 nm, BW 35 nm
Fluorescence filter with holder for installation in compatible microplate readers. Provides excitation in the near-UV at a 360 nm center wavelength with a 35 nm bandwidth; supplied with a holder for secure mounting and intended for fluorescence-based microplate assays.
- Provides 360 nm center wavelength excitation.
- 35 nm bandwidth for selective spectral isolation.
- Supplied with holder for microplate reader installation.
- Designed for fluorescence detection in microplate assays.
- Suitable for assays requiring near-UV excitation and narrow-band filtering.
Molecular Devices Fluorescence Filter, 590 nm, 20 nm bandwidth, Holder
Molecular Devices fluorescence emission bandpass filter with holder designed for narrow emission detection at 590 nm. The filter provides a 20 nm full width passband and installs in a compatible filter holder for microplate reader fluorescence assays.
- Provides a narrow emission passband centered at 590 nm.
- 20 nm full width bandwidth for selective signal detection.
- Supplied mounted in a holder for straightforward instrument installation.
- Optimized for fluorescence emission measurements to improve assay specificity.
- Reduces spectral crosstalk for improved signal-to-noise ratio.
Leica Biosystems Leica Microsystems Filter Cube 405 nm (for Leica fluorescence microscopes)
Filter cube for 405 nm fluorescence imaging. Provides the excitation, dichroic, and emission filtering required for capturing fluorescence excited near 405 nm. Intended as a drop-in accessory for compatible microscope filter turrets to enable bright, high-contrast imaging of blue/violet-excited fluorophores in research and clinical workflows.
- Provides 405 nm excitation band for fluorescence imaging.
- Includes matched excitation, dichroic, and emission filter elements.
- Designed for drop-in installation in compatible filter turret assemblies.
- Suitable for imaging DAPI, Hoechst, and similar 405 nm-excited fluorophores.
- Used in fixed and live-sample fluorescence microscopy workflows.
Molecular Devices Fluorescence Polarization Filters, 360 nm, 35 nm BW
This set includes two fluorescence polarization filters with a holder designed for fluorescence polarization assays in compatible microplate readers. The pair provides polarized excitation at 360 nm with a 35 nm bandwidth and is supplied as vertical and horizontal polarizers to enable polarization measurements.
- Set includes two filters and a single holder.
- Center wavelength 360 nm, with 35 nm bandwidth.
- Polarized vertical and horizontal arrangement for polarization assays.
- Designed for use in compatible microplate readers with filter slide capability.
- Supplied as a matched pair to support accurate fluorescence polarization measurements.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit, 100 Assay Points
Molecular Devices Fluorescence Filter, 400 nm, 30 nm BW, Holder
This fluorescence filter with holder provides an optical band centered at 400 nm with a 30 nm bandwidth for use in microplate-based fluorescence assays, luminescence, and time-resolved fluorescence detection. It serves as an accessory optical element for multimode microplate readers.
- 400 nm center wavelength, 30 nm bandwidth
- Designed for fluorescence, luminescence, and time-resolved fluorescence detection
- Includes mounting holder for installation in standard filter slots
- Intended for use with multimode microplate readers and plate-based assays