Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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MSC
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Sklar Instruments Retractor Sklar Microscopy Surgical 1/EA
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REDSHIFT BIOANALYTICS INC ONE BOX OF CUSTOM 24-WELL AQS3
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One box of custom 24-well AQS3 pro sample plates - 25 plates per box
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HTRF Human Phospho-Cyclin D1 (Thr286) Detection Kit, 500 Assay Points
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Molecular Devices Fluorescence Polarization Filter, Polarized Horizontal, 400-750 nm
The fluorescence polarization filter is an accessory optical filter designed for polarized fluorescence measurements on compatible microplate readers. It provides a horizontal polarization orientation and covers broad visible wavelengths; the filter is supplied with a holder for installation in the instrument's filter cartridge or slide system.
- Polarized horizontal orientation for fluorescence polarization assays.
- Covers 400 nm-750 nm wavelength range.
- Supplied with a holder for installation in the filter cartridge or slide.
- Designed for use in compatible microplate readers to enable polarized fluorescence detection.
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Revvity Health Sciences Inc HTRF Human Phospho-MerTK (Tyr749) Detection Kit, 500 Assay Points
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Mitegen VersaPins All-In-One Tool for Home Source Set-up
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The patented VersaPin for home source allows you to perform several operations needed to prepare your home source for diffraction measurements. The VersaPin performs the role of several tools and will save time while improving the accuracy of your measurements. Applications for the Versapin include: align the sample rotation axis; visualize the X-ray beam; precisely determine beam coordinates; align the sample in the beam; center the beamstop on the beam; calibrate the sample-to-detector distance; calibrate the monochromator energy. The VersaPin is manufactured and sold by MiTeGen under exclusive license from Argonne National Laboratory.
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