Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF XIAP BIR3 Binding Kit Red Ligand, 1 Vial
HTRF XIAP BIR3 Binding Kit Red Ligand, 1 Vial
Electron Microscopy Sciences MAC X-Ray Fluorescence Single Standard Nd
These standards are supplied as 1/4" (33 mm) diameter pressed pellets or metal foils, and are prepared from selected high purity elements and compounds to ensure interference free-spectra. They are suitable for use by experienced spectroscopists as well as staff training. Each of the pressed pellets is supported by a thin-walled aluminum cup which is protected from damage during handling and minimizes the risk of touching the standard materials (some of which are harmful).
Molecular Devices Fluorescence Filter, 535 nm, 25 nm, With Holder
Molecular Devices fluorescence emission filter with integrated holder provides a narrow 535 nm center wavelength and 25 nm bandwidth for selective detection of fluorophores emitting near 535 nm. It is intended for filter-based microplate readers and supports fluorescence and TR-FRET assay detection, including TAMRA-based assays.
- Center wavelength 535 nm.
- Bandwidth 25 nm.
- Includes integrated holder for installation in filter slot.
- Suitable for fluorescence and TR-FRET detection workflows.
- Designed for use in filter-slot multi-mode microplate readers.
Revvity Health Sciences Inc HTRF Human and Mouse Total IKZF1, Control Lysate
HTRF Human and Mouse Total IKZF1, Control Lysate
Revvity Health Sciences Inc HTRF High Performance Human IL-6 Detection Kit, 100,000 Assay Points
HTRF High Performance Human IL-6 Detection Kit, 100,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Phospho-PLC-γ1 (Tyr1253) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Phospho-PLC-γ1 (Tyr1253) Detection Kit, Control Lysate
Molecular Devices Fluorescence Filter, 447 nm, 60 nm, With Holder
Fluorescence donor emission filter for SpectraMax iD5 and iD5e multi-mode microplate readers, centered at 447 nm with a 60 nm bandwidth, supplied with a holder for mounting and optical alignment.
- Isolates emission centered at 447 nm with a 60 nm bandwidth.
- Includes holder for secure mounting in the instrument filter module.
- Compatible with fluorescence and NanoBRET assays on multi-mode microplate readers.
- Improves signal separation for donor emission detection and assay sensitivity.
- Designed for easy installation and reliable optical alignment.
Molecular Devices Emission Filter, 650 nm, 40 nm, FilterMax
Replacement fluorescence emission filter with a 650 nm center wavelength and 40 nm bandwidth, designed for use in Molecular Devices FilterMax F-series multi-mode microplate readers to collect far-red fluorescence emission.
- Center wavelength 650 nm.
- Bandwidth 40 nm for selective far-red detection.
- Designed for installation in filter slide assemblies.
- Enables assays requiring far-red emission collection with reduced background.
- Replacement part for multi-mode microplate reader fluorescence modules.
Electron Microscopy Sciences S10 Stage Counting Grid 0.05 mm Square
Stage graticules are used for the routine calibration of eyepiece patterns, particularly when alternating between objectives on one microscope or when using the same graticule in different microscopes. Their robust construction makes them ideal for student use and for instructional purposes. The scale or grid is centered on a glass disc mounted in a black anodized aluminum slide 75 mm x 24 mm x 2 mm thick. Transmitted light stage slide with 10 x 10 grid of 0.05mm squares.
Revvity Health Sciences Inc HTRF Strep-Tactin d2-Conjugate, 5,000 Assay Points
HTRF Strep-Tactin d2-Conjugate, 5,000 Assay Points
Revvity Health Sciences Inc HTRF CHO HCP Detection Kit, 10,000 Assay Points
HTRF CHO HCP Detection Kit, 10,000 Assay Points