Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Fluorescence Excitation Filter, 590 nm, 20 nm Bandwidth
Replacement fluorescence excitation filter for Molecular Devices FilterMax and compatible multi-mode microplate readers. Provides excitation centered at 590 nm for fluorescence assays and is sold as a replacement filter slide for fluorescence detection.
- Excitation center wavelength: 590 nm.
- Bandwidth: 20 nm.
- Intended for microplate fluorescence detection.
- Fits standard filter slide holders in compatible readers.
Revvity Health Sciences Inc HTRF Human and Mouse Total STAT5 Detection Kit, Control Lysate
HTRF Human and Mouse Total STAT5 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total MET Detection Kit, Control Lysate
HTRF Human Total MET Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total FGFR2 Detection Kit, 50,000 Assay Points
HTRF Human Total FGFR2 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Anti histone H3K4 Me2-EuK mAb conjugate, 10,000 Assay Points
HTRF Anti histone H3K4 Me2-EuK mAb conjugate, 10,000 Assay Points
Revvity Health Sciences Inc HTRF AAV3B Detection Kit, 500 Assay Points
HTRF AAV3B Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Phospho-VAV1 (Tyr174) Detection Kit, Control Lysate
HTRF Phospho-VAV1 (Tyr174) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total TYK2 Detection Kit, 50,000 Assay Points
HTRF Human Total TYK2 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total JAK2 Detection Kit, Control Lysate
HTRF Human Total JAK2 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total IκKα Detection Kit, 500 Assay Points
HTRF Human Total IκKα Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total EGFR DEL19 Detection Kit, Control Lysate
HTRF Human Total EGFR DEL19 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Total Cyclin D1 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total Cyclin D1 Detection Kit, 50,000 Assay Points
Molecular Devices Photometric Filter, 340 nm, FilterMax F3/F5 Compatible
The Molecular Devices AD 340 photometric filter (P/N 6590-0019) is a single-wavelength optical filter centered at 340 nm, supplied as a slide-mounted accessory for multimode microplate readers that accept six-position filter slides. It is intended for absorbance and photometric assays requiring stable, narrowband wavelength selection and reproducible microplate measurements.
- Provides narrowband 340 nm wavelength selection for absorbance assays.
- Slide-mounted thin-film optical filter construction.
- Designed for use with six-position filter slide modules in multimode readers.
- Filter slot dimensions compatible with standard slide limits: excitation 12.2-12.7 mm, emission 17.7-18.0 mm, thickness 5.0-9.0 mm.
- Enables reproducible photometric measurements across microplate assays.