Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 1,000 Assay Points
HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 1,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Lysis Buffer B (5X), 100 mL
AlphaLISA SureFire Ultra Lysis Buffer B (5X), 100 mL
Revvity Health Sciences Inc HTRF Human Total KRAS Detection Kit, 500 Assay Points
HTRF Human Total KRAS Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total SALL4 Detection Kit, 10,000 Assay Points
HTRF Human Total SALL4 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD7 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total BRD7 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human Phospho-CDK1 (Tyr15 & Total) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Multiplex Human Phospho-CDK1 (Tyr15 & Total) Detection Kit, Control Lysate
Molecular Devices Fluorescence Filter, 465 nm, 35 nm, With Holder
Molecular Devices fluorescence band-pass filter supplied with a holder, designed to provide selective optical filtering for fluorescence assays. The filter has a center wavelength of 465 nm and a nominal full width of 35 nm, enabling targeted detection of blue/green fluorophores in multi-mode microplate reader applications.
- Provides a center wavelength of 465 nm for blue/green fluorophore detection.
- 35 nm full width for controlled spectral selection and reduced cross-talk.
- Includes a holder for secure mounting in the instrument filter tray or slide slot.
- Intended for fluorescence detection in multi-mode microplate assays.
- Helps reduce background and improve signal specificity in fluorescence measurements.
Revvity Health Sciences Inc HTRF Human Ataxin 2 Mutant Detection Kit, 500 Assay Points
HTRF Human Ataxin 2 Mutant Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Phospho-RNA polymerase II (Ser5) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Phospho-RNA polymerase II (Ser5) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
Perkin Elmer US LLC Manual Injector Bracket
Magnetic Clip-on Bracket for Manual Injection on FX series LC Instruments.
Revvity Health Sciences Inc HTRF Human and Mouse Total STAT5 Detection Kit, Control Lysate
HTRF Human and Mouse Total STAT5 Detection Kit, Control Lysate
Molecular Devices Fluorescence Polarization Filter, 465 nm, Horizontal
This fluorescence polarization filter provides excitation and detection centered at 465 nm with a 35 nm bandwidth and a horizontal polarization orientation. It is supplied with a holder for installation in compatible multi-mode microplate readers and is intended for fluorescence polarization assays.
- enables fluorescence polarization measurements.
- 465 nm center wavelength, 35 nm bandwidth.
- polarized horizontal orientation for FP assays.
- includes a holder for installation in filter slots.
- designed for use with compatible multi-mode microplate readers.