Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Excitation Filter, 535 nm, 25 nm Bandwidth
This excitation filter provides a 535 nm center wavelength with a 25 nm full-width bandwidth for fluorescence excitation in microplate assays. It is intended for use with multi-mode fluorescence microplate readers to enable selective excitation of fluorophores around 535 nm.
- Center wavelength 535 nm.
- Bandwidth 25 nm full width.
- Excitation filter for fluorescence detection.
- Compatible with multi-mode microplate and ELISA readers.
- Designed for microplate-based fluorescence assays.
Electron Microscopy Sciences Nagoette Double Counting Cell 0.5 mm
Counting cells are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting. All complete with case and 1 pair of cover glasses. The depth of the Nageotte counting is 0.5 mm. For Liquids in Poor content.
Sigma Aldrich Fine Chemicals Biosciences PyrexR low actinic media s1L
Heavy-duty low actinic amber glass bottles protect light-sensitive materials. Include linerless one-piece autoclavable orange PP plug-seal caps (size GL 45) with drip-free pouring rings. Permanent white enamel graduations and marking spots.
Molecular Devices Multi-Mode Microplate Reader, Dual Monochromator, 6-384-Well
The SpectraMax M3 multi-mode microplate reader is a configurable, high-performance instrument for absorbance, fluorescence, and luminescence assays, designed for both research and regulated laboratory environments. It combines tunable optics with flexible plate handling and compliance-capable analysis software.
- Dual monochromator optics, tunable in 1-nm increments
- Detection modes include absorbance, fluorescence, luminescence, fluorescence polarization, and time-resolved assays
- Supports 6- to 384-well microplates, with top and bottom reading options
- High-sensitivity detectors with xenon flash lamp illumination
- Software features secure data acquisition and 21 CFR Part 11-compatible controls
Molecular Devices Microplate Reader, Dual Monochromator, 96/384-Well, Dual-Mode Cuvette
The SpectraMax M2e multi-mode microplate reader provides absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization, and HTRF measurements with flexible plate and cuvette workflows. It features dual monochromators and tunable wavelength selection for assay development and routine screening.
- Measures absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization, and HTRF.
- Compatible with 6-384 well microplates for assay format flexibility.
- Dual monochromators with 1 nm wavelength increments for precise wavelength selection.
- Wavelength coverage for absorbance 200-1000 nm, fluorescence excitation 250-850 nm, and emission 250-850 nm.
- Includes a dual-mode cuvette port for top and bottom read formats.
- Operates from 100-240 Vac, 50/60 Hz, 3 A power input.
- Designed for integration with automation and robotic systems.