Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Phospho-MerTK (Tyr749) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-MerTK (Tyr749) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total VEGFR2 Detection Kit, 500 Assay Points
HTRF Human Total VEGFR2 Detection Kit, 500 Assay Points
Molecular Devices Fluorescence Emission Filter, 430 nm, 35 nm Bandwidth
Molecular Devices fluorescence emission filter providing a narrow emission band centered at 430 nm for fluorescence detection. The filter has a 35 nm full width at half maximum (FWHM) bandwidth and is intended for use in filter-based fluorescence detection systems such as microplate readers.
- Provides emission bandpass centered at 430 nm.
- 35 nm full width at half maximum bandwidth.
- Designed for fluorescence detection applications.
- Suitable for use in filter-based microplate readers and optical filter assemblies.
Revvity Health Sciences Inc HTRF Human Total p21 Detection Kit, 50,000 Assay Points
HTRF Human Total p21 Detection Kit, 50,000 Assay Points
Molecular Devices Absorbance Filter, 510 nm, 8 nm Bandwidth
The Molecular Devices absorbance photometric filter is a narrowband 510 nm filter supplied as a slide element for installation into compatible microplate readers, enabling precise absorbance measurements and improved assay specificity.
- Designed for absorbance measurements at 510 nm.
- 8 nm bandwidth for narrowband detection and reduced spectral overlap.
- Slide-mounted format for easy installation into filter assemblies.
- High-quality optical coatings to improve signal-to-noise ratio.
- Suitable for a range of optical assays requiring 510 nm measurements.
Molecular Devices Fluorescence Excitation Filter, 525 nm, 45 nm Bandwidth
The Molecular Devices 525 nm fluorescence excitation filter (6590-0052) is a 45 nm bandpass excitation filter designed for fluorescence assays requiring green-range illumination. It is intended for use with multi-mode microplate readers that accept filter slide carriers and provides selective excitation centered at 525 nm.
- Filter type: fluorescence excitation filter.
- Center wavelength: 525 nm.
- Bandwidth: 45 nm.
- Mounting format: filter slide compatible with multi-filter slide carriers.
- Application: optimized for green-range fluorescence assays.
Revvity Health Sciences Inc HTRF Human Androgen Receptor Detection Kit, 10,000 Assay Points
HTRF Human Androgen Receptor Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total HSP27 Detection Kit, 500 Assay Points
HTRF Human Total HSP27 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total RET Detection Kit, 500 Assay Points
HTRF Human & Mouse Total RET Detection Kit, 500 Assay Points
Molecular Devices Fluorescence Polarization Filter, Polarized Horizontal, 300-750 nm
Molecular Devices polarized horizontal fluorescence polarization filter for use in fluorescence polarization assays on compatible multi-mode microplate readers. The filter transmits across a broad UV-visible range and installs in a standard filter slide to enable polarized detection.
- Polarized horizontal orientation.
- Wavelength range 300-750 nm.
- Enables fluorescence polarization measurements.
- Installs in a standard filter slide for compatible multi-mode readers.
- Broad UV-visible transmission for diverse fluorophores.
Revvity Health Sciences Inc AlphaLISA KRAS WT / SOS1 Binding Kit, 5,000 Assay Points
AlphaLISA KRAS WT / SOS1 Binding Kit, 5,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total SMARCA-2 Detection Kit, 500 Assay Points
HTRF Human Total SMARCA-2 Detection Kit, 500 Assay Points
Molecular Devices Photometric Filter, 405 nm, 10 nm Bandwidth
AD 340 photometric absorbance filter for narrowband absorbance measurements at 405 nm. Designed as a replacement or accessory optical filter for absorbance channels in microplate reader assays, it provides a focused transmission band for reliable endpoint and kinetic measurements.
- Center wavelength: 405 nm.
- Bandwidth: 10 nm.
- Measurement type: Absorbance (photometric).
- Typical applications: Endpoint and kinetic microplate assays.
- Form factor: Optical bandpass photometric filter.
Molecular Devices Fluorescence Polarization Filter, 535 nm, 25 nm, Vertical
Fluorescence polarization filter optimized for fluorescence polarization assays. The filter has a 535 nm center wavelength with a 25 nm bandwidth and a vertical polarization orientation. It includes a holder for mounting in compatible microplate readers and is intended for use with FP measurement configurations.
- Optimized for fluorescence polarization measurements.
- Center wavelength 535 nm, bandwidth 25 nm.
- Vertical polarization orientation for polarization-based assays.
- Supplied with a holder for secure mounting in reader filter positions.
- Compatible with SpectraMax and similar microplate readers that accept filter holders.
Revvity Health Sciences Inc HTRF Human IRF5 Detection Kit, 500 Assay Points
HTRF Human IRF5 Detection Kit, 500 Assay Points