Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Time-Resolved Filter, 350 nm, 60 nm Bandwidth
This time-resolved excitation filter selects short-wavelength excitation light centered at 350 nm with a 60 nm full width, for use in time-resolved fluorescence (TRF) assays on compatible filter-based microplate readers. It is intended for applications that require narrow-band excitation around 350 nm for TRF measurements.
- Time-resolved excitation filter.
- Center wavelength 350 nm.
- Bandwidth 60 nm (full width).
- Optimized for time-resolved fluorescence assays.
- Compatible with SpectraMax iD5 filter module and similar readers.
- Fits standard filter holder assemblies in compatible readers.
Revvity Health Sciences Inc HTRF cIAP1 BIR2 Binding Kit, 500 Assay Points
HTRF cIAP1 BIR2 Binding Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Strep-Tactin Tb-Conjugate, 20,000 Assay Points
HTRF Strep-Tactin Tb-Conjugate, 20,000 Assay Points
Electron Microscopy Sciences Nagoette Double Counting Cell 0.5 mm
Counting cells are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting. All complete with case and 1 pair of cover glasses. The depth of the Nageotte counting is 0.5 mm. For Liquids in Poor content.
THERMO SCI LSG 3P Robotic Tray including adapter for plate without lid
Robotic Tray including adapter for plate without lid
Revvity Health Sciences Inc HTRF Human Frataxin Detection Kit, 10,000 Assay Points
HTRF Human Frataxin Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total EGFR DEL19 Detection Kit, 50,000 Assay Points
HTRF Human Total EGFR DEL19 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total PR Detection Kit, 10,000 Assay Points
HTRF Human Total PR Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total PINK3 Detection Kit, 50,000 Assay Points
HTRF Human Total PINK3 Detection Kit, 50,000 Assay Points
Molecular Devices Fluorescence Filter, Holder, 360 nm, BW 35 nm
Fluorescence filter with holder for installation in compatible microplate readers. Provides excitation in the near-UV at a 360 nm center wavelength with a 35 nm bandwidth; supplied with a holder for secure mounting and intended for fluorescence-based microplate assays.
- Provides 360 nm center wavelength excitation.
- 35 nm bandwidth for selective spectral isolation.
- Supplied with holder for microplate reader installation.
- Designed for fluorescence detection in microplate assays.
- Suitable for assays requiring near-UV excitation and narrow-band filtering.
Leica Biosystems Leica Microsystems Filter Cube 405 nm (for Leica fluorescence microscopes)
Filter cube for 405 nm fluorescence imaging. Provides the excitation, dichroic, and emission filtering required for capturing fluorescence excited near 405 nm. Intended as a drop-in accessory for compatible microscope filter turrets to enable bright, high-contrast imaging of blue/violet-excited fluorophores in research and clinical workflows.
- Provides 405 nm excitation band for fluorescence imaging.
- Includes matched excitation, dichroic, and emission filter elements.
- Designed for drop-in installation in compatible filter turret assemblies.
- Suitable for imaging DAPI, Hoechst, and similar 405 nm-excited fluorophores.
- Used in fixed and live-sample fluorescence microscopy workflows.
Molecular Devices Fluorescence Polarization Filters, 360 nm, 35 nm BW
This set includes two fluorescence polarization filters with a holder designed for fluorescence polarization assays in compatible microplate readers. The pair provides polarized excitation at 360 nm with a 35 nm bandwidth and is supplied as vertical and horizontal polarizers to enable polarization measurements.
- Set includes two filters and a single holder.
- Center wavelength 360 nm, with 35 nm bandwidth.
- Polarized vertical and horizontal arrangement for polarization assays.
- Designed for use in compatible microplate readers with filter slide capability.
- Supplied as a matched pair to support accurate fluorescence polarization measurements.
Revvity Health Sciences Inc HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 10,000 Assay Points
Molecular Devices Photometric Filter, 540 nm, 8 nm Bandwidth
Molecular Devices AD 340 photometric filter provides a narrow-band absorbance measurement centered at 540 nm for compatible multimode microplate readers. It is intended as a replacement or accessory filter for routine absorbance assays and instrument maintenance.
- Provides a 540 nm center wavelength for absorbance measurements.
- 8 nm full width at half maximum (bandwidth) for narrow-band detection.
- Bandpass design reduces out-of-band signal and improves assay specificity.
- Designed for use with compatible multimode microplate readers; verify instrument compatibility before ordering.
- Packaged as a single replacement filter for instrument maintenance and routine swaps.