Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Androgen Receptor Full Length Detection Kit, 500 Assay Points
HTRF Human Androgen Receptor Full Length Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total CHK-1 Detection Kit, Control Lysate
HTRF Human & Mouse Total CHK-1 Detection Kit, Control Lysate
Molecular Devices Absorbance Filter, 414 nm, 10 nm, FilterMax
The FilterMax absorbance photometric filter provides a narrowband 414 nm detection channel with a 10 nm bandwidth for photometric and absorbance assays on compatible multimode microplate readers. It is an optical replacement accessory intended to restore or extend the absorbance measurement capability of readers that accept replaceable filters.
- Center wavelength 414 nm, bandwidth 10 nm.
- Designed for narrowband absorbance and photometric measurements.
- Intended as a replacement optical filter for compatible multimode microplate readers.
- Supports absorbance-based assays that require a 414 nm detection channel.
- Sold as a single replacement filter for installation by qualified service personnel.
Molecular Devices Fluorescence Excitation Filter, 465 nm, 35 nm, FilterMax
Replacement fluorescence excitation filter designed for use with Molecular Devices FilterMax family microplate readers. The filter is centered at 465 nm with a 35 nm optical bandwidth to selectively transmit excitation light for fluorophores with peak absorption near this wavelength.
- Excitation filter for fluorescence microplate measurement.
- Center wavelength 465 nm.
- 35 nm optical bandwidth (FWHM).
- Fits standard FilterMax filter slide assemblies.
- Used for fluorescence intensity and polarization assays.
Molecular Devices Fluorescence Filter Slide, 485/20 nm, 525/30 nm
Molecular Devices fluorescence filter slide provides narrow bandpass excitation and emission filters for fluorescence detection in compatible microplate readers. The slide features a 485/20 nm excitation filter and a 525/30 nm emission filter to enable sensitive measurement of visible-range fluorophores.
- Excitation bandpass 485/20 nm.
- Emission bandpass 525/30 nm.
- Designed for fluorescence detection in microplate readers.
- Serves as a replaceable filter slide for reader configurations.
- Supports common visible-range fluorophores for assay flexibility.
Alkali Scientific ASI™ Black Confocal Dish 35 x10mm, Easy-Grip, Adhesion Type, Sterile, Treated (Positive-Charged/ Surface Coated), 50/Cs, 22 x 22 x 0.13mm, Hole 3.14mm, PS/Flux
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products:Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy Bottom materials: Glass/FLux (no surface treatment for glass bottom) Coverslip: #1.5 Dish color: Clear/Black Dish size: 35 Ø Hole size: 13 Ø / 20 Ø / 25Ø Non – pyrogenic Non – cytotoxic DNase / RNase – free Human DNA – free
Revvity Health Sciences Inc HTRF EPIgeneous Binding Domain B Detection Kit, 10,000 Assay Points
HTRF EPIgeneous Binding Domain B Detection Kit, 10,000 Assay Points
Molecular Devices Reader Cable, MAC-Series 1, Emax and Vmax Compatible
Molecular Devices replacement cable designed to connect MAC-Series 1 readers to compatible Emax and Vmax microplate readers for instrument control and data transfer.
- Replacement interconnect cable for legacy microplate readers.
- Enables data and control signal transfer between instruments.
- Plug-and-play design for straightforward installation.
- Supplied as a single unit for spare parts inventory.
Revvity Health Sciences Inc HTRF Human & Mouse Total HDAC5 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total HDAC5 Detection Kit, 10,000 Assay Points
Enterprise Technology Solutions Customizable Print Dividers
Customizable Print-On Dividers Unpunched 5-Tab 11 x 8 5 White 5 Sets
Molecular Devices Enhanced TRF Module, Time-Resolved Fluorescence, SpectraMax iD5 Compatible
The Enhanced TRF Module expands time-resolved fluorescence performance of SpectraMax iD5 and iD5e microplate readers, enabling TRF, TR-FRET, HTRF, IMAP, and ScanLater western blot reads with improved sensitivity for lanthanide assays.
- Improved sensitivity for Europium detection down to 10 fM.
- Enables TRF, TR-FRET, and HTRF assay read modes.
- Supports ScanLater western blot TRF reads.
- Operates with configurable flash intervals for time-resolved reads.
- Compatible with exchangeable excitation and emission filter slides sold separately.
- Requires compatible data acquisition software for some TR-FRET read modes.
Revvity Health Sciences Inc HTRF Human & Mouse Total Cyclin D1 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total Cyclin D1 Detection Kit, 50,000 Assay Points