Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Agilent BioTek Cytation C10 Confocal Imaging Reader C10IPHC2-SN PROMO
Includes a confocal microscope with 60 μm Nipkow spinning disk and either 40 μm spinning disk or 60 μm deep-sectioning disk; widefield inverted microscope for fluorescence, brightfield, color brightfield, phase contrast, and water immersion imaging with Hamamatsu sCMOS camera
Agilent BioTek Lamp Replacement Kit for ELx808 Ultra Microplate Reader
Accessory for use with ELx808 Ultra Microplate Readers.
Agilent BioTek Cytation C10 Confocal Imaging Reader C10MIPW-SN PROMO
Includes a widefield inverted microscope for fluorescence, brightfield, color brightfield, phase contrast, and water-immersion imaging with Sony CMOS camera; monochromator-based multimode plate reader; upgradable to add confocal microscope
Agilent BioTek Cytation C10 Confocal Imaging Reader C10MIPWC-SN PROMO
Includes a confocal microscope with 60 μm Nipkow disk, widefield inverted microscope for fluorescence, brightfield, color brightfield, phase contrast, and water-immersion imaging with Sony CMOS camera; monochromator-based multimode plate reader
Agilent BioTek Cytation C10 Confocal Imaging Reader C10IPWC-SN PROMO
Includes a confocal microscope with 60 μm Nipkow spinning disk, widefield inverted microscope for fluorescence, brightfield, color brightfield, phase contrast, and water-immersion imaging with Sony CMOS camera
Agilent BioTek Cytation C10 Confocal Imaging Reader C10IPW-SN PROMO
Includes a widefield inverted microscope for fluorescence, brightfield, color brightfield, phase contrast, and water-immersion imaging with Sony CMOS camera; upgradable to add confocal microscope
