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Ability to encode cell and measure cell proliferation and using the violet laser.Multiplex with other MultiCyt kits like Cell Membrane Integrity or Qbeads for richer content.Dyes are safe and non-toxic to the cells.Covalently attaches to intracellular proteins enabling its long-term retention.PrincipleProliferation Use: The principle of dye dilution. Cell proliferation is detected and quantified based on the halving or “dilution” of the loaded dye after each round of cellular division. Through subsequent cell divisions daughter cells retain approximately half the fluorescent label of the parent cells.Encoding Use: Multiple cell populations are stained with different intensities of dye distinguish up to 4 cell populations Bright Mid Dim and Unstained . Target cells are stained with different concentrations of V/Blue encoding dye in order to discriminate the different cell populations.
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The BD Biosciences HTS Kit Parts Replacement is essential for maintaining high throughput sample acquisition in BD FACSCanto and FACSCanto II flow cytometers. It enhances laboratory efficiency by enabling seamless conversion between tube-based and multi-well plate-based sampling. These replacement parts are manufactured under Good Manufacturing Practices (GMP), ensuring reliable performance and consistent results for research applications.
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The purpose of this kit is to provide the necessary reagents needed to prepare the BD Rhapsody™ Cartridges, extract RNA and antibody oligos bound to the cells, and capture them on BD Rhapsody™ Cell Capture Beads.