Classical and Basal Cell Culture Media
NEOGEN Buffered Peptone Water (BPW), 5 kg
Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp. from food and is not intended for use in the diagnosis of disease or other conditions in humans. Edel and Kamelmacher found that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to Salmonella spp. Preenrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Lactose Broth is frequently used for this purpose, but it may be detrimental to recovering Salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods. For more information visit https://www.neogen.com/solutions/Microbiology/buffered-peptone-water
NEOGEN Campylobacter Enrichment Broth (Bolton Broth), 500 g
Campylobacter Enrichment Broth (Bolton Broth) is used with antimicrobics for the selective enrichment of Campylobacter spp. Campylobacter Enrichment Broth (Bolton Broth) is not intended for use in the diagnosis of disease or other conditions in humans. Campylobacter spp. are microaerophilic, very small, curved, thin, Gram-negative rods. Microaerophilic organisms have a tendency to be more sensitive to toxic forms of oxygen. Campylobacter Enrichment Broth (Bolton), along with nutritional ingredients, contains compounds which enhance the aerotolerance of microaerophilic bacteria by suppressing the toxic form of oxygen. Campylobacter Enrichment Broth (Bolton) is recommended in food testing. Blood-Free Campylobacter Enrichment Broth, Bolton Broth (2X Concentration) is described by the USDA.For more information visit https://www.neogen.com/solutions/Microbiology/campylobacter-enrichment-broth-bolton
NEOGEN Buffered Listeria Enrichment Broth, 5 kg
Buffered Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. Evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-listeria-enrichment-broth
NEOGEN Casman Medium Base, 500 g
Casman Medium Base is used with blood for the isolation of Haemophilus influenzae, Neisseria gonorrhoeae and other fastidious microorganisms in a laboratory setting. Casman Medium Base is not intended for use in the diagnosis of disease or other conditions in humans.In 1947, Casman described an infusion-free medium enriched with 5% blood for fastidious microorganisms incubated anaerobically. This medium replaced formulas containing fresh meat infusion and heated blood. Casman adjusted the formula after experiments revealed nicotinamide disrupted the action of a blood enzyme that inactivates V factor (NAD). The concentration of nicotinamide was lowered to support growth of Neisseria spp.For more information visit https://www.neogen.com/solutions/Microbiology/casman-medium-base
NEOGEN Violet Red Bile Glucose Agar (ISO), 500 g
Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods and is not intended for use in the diagnosis of disease or other conditions in humans.Formulated according to ISO 21528:2017 (all parts), VRBGA is a modification of Violet Red Bile Lactose Agar (NCM0089) introduced by Mossel in 1978. VRBA contains lactose which is fermented by members of the coli/ aerogenes group, this medium gives a ‘coliform’ count. The VRBGA formula modification replaces lactose with glucose. Glucose is fermented by all members of the Enterobacteriaceae thus gives a presumptive Enterobacteriaceae count. Bile salts and crystal violet are used to inhibit Gram positive and non-enteric organisms. The overlay procedure ensures anaerobic conditions and suppresses the growth of non-fermentative Gram negative bacteria.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-glucose-agar-iso
NEOGEN Letheen Broth with Tween, Modified, 500 g
Letheen Broth with Tween, Modified is used for the recovery of microorganisms from cosmetics in a laboratory setting and is not intended for use in the diagnosis of disease or other conditions in humans.In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical as disinfectant residues can result in a false negative (no-growth) test result.Letheen Broth with Tween, Modified is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Broth Base.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-broth-with-tween-modified
NEOGEN m-HPC Agar, 500 g
m-HPC Agar is used for the enumeration of heterotrophic organisms in water by the membrane filtration technique in a laboratory setting. m-HPC Agar is not intended for use in the diagnosis of disease or other conditions in humans.m-HPC Agar, also known as m-Heterotrophic Plate Count Agar, was developed by Taylor and Geldreich in 1979. Monitoring the levels of heterotrophic bacteria, those microorganisms that can grow on minimal organic nutrients, is currently performed to indicate problems with treatment methods within distribution systems. There is evidence that heterotrophic bacteria are not hazardous to healthy individuals, but may be opportunistic pathogens capable of infecting individuals with impaired immune systems.m-HPC Agar is used in standard methods for the detection of heterotrophic bacteria using the membrane filtration technique.For more information visit https://www.neogen.com/solutions/Microbiology/m-hpc-agar
NEOGEN Buffered Listeria Enrichment Broth Base, 5 kg
Buffered Listeria Enrichment Broth Base is used with supplements for selective enrichment of Listeria spp. in foods and is not intended for use in the diagnosis of human disease.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-listeria-enrichment-broth-base
NEOGEN Urea Agar Base (Christensen), 500 g
Urea Agar Base is used with agar for the differentiation of microorganisms on the basis of urease production in a laboratory setting. Urea Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Christensen devised a urea agar medium containing peptone and dextrose that had a reduced buffer content. The medium supported the vigorous growth of many Gram-negative, enteric bacilli and readily permitted observation of urease production. Urea Agar Base may be used as a screening medium (along with Triple Sugar Iron Agar) for the selection of Salmonella and Shigella cultures for serologic classification. Urea Agar Base is also used to detect production of urease by yeast. Urease production is an important differential test in microbiology and outlined in standard methods.For more information visit https://www.neogen.com/solutions/Microbiology/urea-agar-base-christensen
NEOGEN Sugar Free Agar, 500 g
Sugar Free Agar is used for the enumeration of psychrotrophic and mesophilic Gram-negative rods, and is not intended for use in the diagnosis of disease or other conditions in humans.A formula described by the International Dairy Federation for the enumeration of psychrotrophic and mesophilic Gram negative rods in butter and other dairy products. The Gram negative rods are able to deaminate proteins as a carbon source, whilst some enterococci are inhibited by this formula. The medium conforms to the formulation of the International Dairy Federation (I.D.F.).Formula:Gelatin Peptone - 7.5 g/LTryptone - 7.5 g/LSodium Chloride - 5.0 g/LAgar - 14.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/sugar-free-agar
NEOGEN Buffered Listeria Enrichment Broth Base, 500 g
Buffered Listeria Enrichment Broth Base is used with supplements for selective enrichment of Listeria spp. in foods and is not intended for use in the diagnosis of human disease.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-listeria-enrichment-broth-base
NEOGEN Pseudomonas Isolation Agar, 500 g
Pseudomonas Isolation Agar is used for the isolation of Pseudomonas aeruginosa and other Pseudomonas spp. in a laboratory setting. Pseudomonas Isolation Agar is not intended for use in the diagnosis of disease or other conditions in humans. Pseudomonas Isolation Agar is based on Medium A described by King, Ward, and Raney. This medium is very useful for isolating Pseudomonas spp. Pseudomonas Isolation Agar includes Irgasan®, a potent broad spectrum antimicrobial not active against Pseudomonas spp. This medium is selective and formulated to enhanced formation of blue or blue-green pyocyanin pigment by Pseudomonas aeruginosa. The pigment diffuses into the medium surrounding growth.For more information visit https://www.neogen.com/solutions/Microbiology/pseudomonas-isolation-agar
NEOGEN Agar, Technical, 500 g
Agar, Technical is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Technical is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to US Pharmacopeia (USP).Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperatures for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.For more information visit https://www.neogen.com/solutions/Microbiology/agar-technical
NEOGEN Skim Milk Powder, 500 g
This item has a minimum order quantity of 5 per supplier requirements
Skim Milk Powder is used when preparing microbiology culture media in a laboratory setting. Milk Powder is not intended for use in the diagnosis of disease or other conditions in humans. A bacteriological grade of thermophile free spray dried skim milk. Used in Milk Plate Count Agar NCM0119 and in media for diagnostic tests involving the digestion or coagulation of casein and the fermentation of lactose. Recommended working concentration 10%.For more information visit https://www.neogen.com/solutions/Microbiology/skim-milk-powder
NEOGEN Schaedler Agar, 500 g
Schaedler Agar is used for the cultivation of anaerobic microorganisms in a laboratory setting. Schaedler Agar is not intended for use in the diagnosis of disease or other conditions in humans. Survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature. Schaedler Agar is suitable for standard procedures used in cultivating anaerobic bacteria. Schaedler Agar is prepared according to the formulation described by Schaedler, Dubos, and Costello, and modified by Mata, Carrillo, and Villatoro. Modifications include reduced dextrose to avoid interference with hemolytic reactions, reduced yeast extract to avoid darkening of the medium, and adjusted sodium chloride and nitrogen concentrations.For more information visit https://www.neogen.com/solutions/Microbiology/schaedler-agar